Urine DNA Isolation Kit (Slurry Format)

Fast, reliable isolation of urine DNA from 3 mL to 25 mL samples

  • Unlike other methods, Norgen’s Urine DNA Isolation Kit (Slurry Method) does not require any additional urine concentrating devices
  • Fast processing time
  • Purify both genomic and apoptotic DNA with one protocol
  • Isolate DNA from 3 mL to 25 mL of urine
  • Allows for purification of viral DNA from urine also
  • Available in Spin Column and 96-well format
Items Cat. # Size
Urine DNA Isolation Kit (Slurry Format) 48800 50 Preps
Urine DNA Isolation 96-Well Kit (Slurry Format) 27100 1 x 96-well plate

Urine DNA Isolation Kit (Slurry Format)

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 3 mL to 25 mL. Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 - 250 bp; derived from the circulation) is effectively isolated and purified with a rapid and convenient spin column protocol. Multiple samples can be processed in 45 minutes. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications such as PCR, qPCR, DNA fingerprinting, methylation studies and more. This kit can also be used to isolate DNA from a broad range of viruses.

This kit is fully compatible with Norgen's Urine Collection and Preservation Tubes.

Background

Many advantages favour the use of urinary DNA for cancer biomarker discovery and diagnosis of different pathogens over the use of blood and tissue samples. DNA found in urine can be divided into 2 basic categories. The larger genomic DNA (gDNA) species is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid. Both types of DNA can be isolated reliably using this kit.

 

Figure 3. Highly Sensitive Isolation of Viral DNA

Kit Specifications - Spin Column
Minimum Urine Input 3 mL
Maximum Urine Input 25 mL
Time to Complete 10 Purifications < 30 minutes
Size of Urine DNA Purified Large (> 1 kb) and
small (150-250 bp)
Kit Specifications - 96-well
Urine Input Volume
3 - 25 mL
Elution Volume
1st Elution 100 μL
2nd Elution 100 μL
Size of DNA Purified
Large (> 1 kb) and
small (150-250 bp)
Time to Complete 96 Purifications
< 1 hour

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25oC) for up to 1 year without showing any reduction in performance. It is recommended to warm up Solution A and Solution B for 20 minutes at 60oC if any salt precipitation is observed.

 

 

Kit Components
Component Cat. 48800 (50 preps)
Slurry B1 22 mL
Lysis Buffer A 30 mL
Wash Solution A 38 mL
Elution Buffer B 15 mL
Mini Filter Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1