Urine DNA Isolation Kit for Exfoliated Cells or Bacteria

For the rapid and efficient purification of DNA from exfoliated cells or bacteria in urine

  • Isolate genomic DNA from either exfoliated cells or bacteria found in urine
  • Isolate and detect genomic DNA from as little as 1 mL of urine and up to 50 mL urine
  • High quality DNA for sensitive applications
  • Rapid processing time
Items Cat. # Size
Urine DNA Isolation Kit for Exfoliated Cells or Bacteria 47050 50 preps

Urine DNA Isolation Kit for Exfoliated Cells or Bacteria

This kit provides rapid spin column purification of either: 1) human genomic DNA from exfoliated cells that have been shed into the urine from the urinary tract; or 2) bacterial genomic DNA from urine samples. It can process volumes between 1 to 50 mL of urine. The kit allows for the isolation of genomic DNA from both Gram-negative and Gram-positive bacteria or exfoliated cells. Multiple samples can be processed in 45 minutes (including a 30 minute incubation). Salts, metabolic wastes and proteins are removed to yield high quality DNA compatible with any application such as PCR, qPCR, methylation studies, sequencing and more.

Healthy humans generally have < 10, 000 CFU of bacteria per mL of urine, and this kit is sufficiently sensitive to isolate and detect DNA from even this minimal amount of bacteria

Figure 3.  Isolation of Gram-Positive and Gram-Negative Bacteria

Kit Specifications
Minimum Urine Input
1 mL
Maximum Urine Input
50 mL
Time to Complete 10 Purifications
10 minutes
(Plus a 30 minute incubation - Bacteria)
(Plus a 15 minute incubation - Exfoliated)

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. This kit contains a ready-to-use Proteinase K solution which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

 

 

Kit Components
Component Cat. 47050 (50 preps)
Resuspension Solution A 12 mL
Lysis Buffer B 40 mL
Wash Soltuion A 20 mL
Elution Buffer B 12 mL
Proteinase K in Storage Buffer 8 mL
Micro Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1
Title The Role of Urinary Cathelicidin (LL-37) and Human β-defensin 1 (hBD-1) in Uncomplicated Escherichia coli Urinary Tract Infections.
Journal Infection and Immunity. 2014.
Authors Nielsen KL, Dynesen P, Larsen P, Jakobsen L, Andersen PS, Frimodt-Møller N.