Total RNA Purification Plus Micro Kit

Rapid purification of total RNA - including microRNA - from small input amounts

  • Extract high quality & purity total RNA including miRNA
  • Elute extracted total RNA in smaller volume (20 µL)
  • No phenol step required - isolate all RNA in one fraction
  • Genomic DNA Removal Column for efficient elimination of gDNA
  • Bind & elute all RNA irrespective of size or GC content, without bias.
  • Efficiently extract small RNA irrespective of GC content
  • Very sensitive & linear down to a few cells without the need for carrier RNA
  • Convenient & fast spin column format
Items Cat. # Size
Total RNA Purification Plus Micro Kit 48500 50 Preps


Total RNA Purification Plus Micro Kit

This kit extracts total RNA from low cell number samples and elutes in a convenient 20 µL elution suitable for a number of downstream applications. In addition gDNA is removed efficiently with a gDNA removal column and therefore no DNase step is required.

Efficiently extract total RNA from a range of samples including small input of cells, small tissues, needle biopsies, LCM, CTC and other low cell number samples. Extract high quality and purity RNA with excellent RIN values and A260/A280suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

 

 

Kit Specifications
Maximum Column Binding Capacity
35 μg
Maximum Column Loading Volume
650 μL
Size of RNA Purified

All sizes, including
small RNA (< 200 nt)

Maximum Amount of Starting Material:
Animal Cells
Animal Tissues
Laser-Captured Microdissection (LCM)
5 x 105 cells
3 mg (for most tissues*)
Up to 5 x 105 cells
Time to Complete 10 Purifications
22 minutes
Average Yields*
HeLa Cells (1 x 105 cells)
1.5 μg

* for fibrous tissue, an additional Proteinase K treatment is required

Kit Components
Component Cat. 48500 (50 preps)
Buffer RL 40 mL
Wash Solution A 38 mL
Elution Solution A 6 mL
Genomic DNA Removal Column 50
RNA Purification Micro Column 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

    

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Journal American Journal of Physiology - Cell Physiology. 2014.
Authors Michael W. Nestor, Samson Jacob, Bruce Sun, Deborah Pre, Andrew A. Sproul, Seong Im Hong, Chris Woodard, Matthew Zimmer, Vorapin Chinchalongporn, Ottavio Arancio, Scott A. Noggle.
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Journal Neuropeptides2014.
Authors Betty McConn, Guoqing Wang, Jiaqing Yi, Elizabeth R. Gilbert, Tomohiro Osugi, Takayoshi Ubuka, Kazuyoshi Tsutsui, Vishwajit S. Chowdhury, Mitsuhiro Furuse, Mark A. Cline.
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Journal Neuropeptides. 2014.
Authors Jacob D. Mace, Tetsuya Tachibana, Guoqing Wang, Brandon A. Newmyer, Evin Guilliams, Elizabeth R. Gilbert, Mark A. Cline.
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Journal FASEB Journal. 2014.
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