Stool Nucleic Acid Isolation Kit

A convenient and rapid method to isolate total DNA and RNA from fresh, frozen and preserved stool samples

  • Simultaneous isolation of both host and microbial DNA and RNA
  • Eliminates PCR inhibitors including humic acids
  • High quality total RNA and DNA for sensitive downstream applications

Figure 1.  Total RNA Profile

Items Cat. # Size
Stool Nucleic Acid Isolation Kit 45600 50 preps

Stool Nucleic Acid Isolation Kit

This kit provides a rapid spin column method to isolate total DNA and RNA from fresh or frozen stool samples, as well as from stool collected using Norgen's Stool Nucleic Acid Collection and Transport Tubes. The universal protocol conveniently allows for the isolation of total genomic DNA and total RNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and provides consistent, high yields of RNA and DNA.

The purified DNA and RNA are of the highest quality and are fully compatible with downstream PCR applications, as all humic acid substances and other PCR inhibitors are removed during the isolation.

Figure 4.  Detection of Human DNA


Kit Specifications
Maximum Stool Input
200 mg
Type of Stool Processed
Frozen and fresh stool
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Time to Complete 10 Purifications
30 minutes

Storage Conditions and Product Stability 
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.



Kit Components
Component Cat. 45600 (50 preps)
Lysis Solution 60 mL
Binding Solution 6 mL
Wash Solution 22 mL
Elution Buffer 6 mL
Bead Tube 50
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1