Stool DNA Isolation Kit

For the rapid and simple purification of bacterial and host DNA from stool and fecal samples

  • Simultaneous isolation of both host DNA and microbial DNA (universal protocol)
  • Eliminates PCR inhibitors including humic acids
  • Fully compatible with Norgen's Stool Nucleic Acid Collection and Transport Tubes
  • High quality DNA for sensitive downstream applications including PCR, qPCR, Sequencing and microarray
     
    Figure 1.  Higher Yields of DNA than Competitor Z
Items Cat. # Size
Stool DNA Isolation Kit 27600 50 preps

Stool DNA Isolation Kit

This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’sStool Nucleic Acid Collection and Transport Tubes (Cat. 45630). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and other inhibitors using Bead Tubes and a combination of chemical and physical homogenization and lysis, without the need of any phenol-chloroform extractions. A simple and rapid spin column procedure is then used to further purify the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, Sequencing and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.

Kit Specifications
Maximum Stool Input
200 mg
Type of Stool Processed
Frozen, fresh or preserved stool
Format Spin Column
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Elution Volume 50 μL
Time to Complete 10 Purifications
30 minutes
Applications

PCR, qPCR, Southern Blot Analysis,
Sequencing, Microarray Analysis.

Storage Conditions and Product Stability 
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.

 

 

Kit Components
Component Cat. 27600 (50 preps)
Lysis Buffer L 60 mL
Lysis Additive A 6 mL
Binding Buffer I 7 mL
Buffer SK 30 mL
Wash Solution A 18 mL
Elution Buffer B 8 mL
Bead Tube 50
Mini Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1
Title Cryptosporidiosis outbreak in a child day-care center caused by an unusual Cryptosporidium hominis subtype
Journal Enfermedades Infecciosas y Microbiología Clínica2015.
Authors Pilar Goñi, Diego Almagro-Nievas, Joanna Cieloszyk, Silvia Lóbez, José María Navarro-Marí, José Gutiérrez-Fernández
Title Detection of Campylobacter in human faecal samples in Fiji
Journal Western Pacific Surveillance and Response Journal2014.
Authors Aruna Devi, Jenny Wilkinson, Timothy Mahony and Thiru Vanniasinkam
Title How to make DNA count: DNA-based diagnostic tools in veterinary parasitology.
Journal Veterinary Parasitology2011.
Authors Hunt PW, Lello J.