Sputum DNA Isolation Kit

For the rapid purification of high quality DNA from sputum samples

  • Fast and easy processing using a spin column format
  • DNA can be isolated and detected from as little as 100 µL of sputum
  • Includes Sputum Liquifier to reduce viscosity and facilitate DNA isolation
  • Effective removal of PCR inhibitors
  • Fully compatible with Norgen’s Sputum DNA Collection, Preservation and Isolation Kit (Cat#RU46100).

 

Figure 1.  Consistent Isolation of Sputum DNA

Items Cat. # Size
Sputum DNA Isolation Kit 46200 25 Preps

Sputum DNA Isolation Kit

This kit constitutes an all-in-one system for the rapid isolation of DNA from sputum samples. It allows for the isolation of bacterial or eukaryotic DNA from the sputum samples using a convenient spin column format. The kit includes all the reagents needed to process sputum DNA including a sputum liquifier to reduce viscosity and facilitate DNA isolation. The protocol can be completed in 30 minutes. Purified DNA is of the highest quality and free from inhibitors, and can be used in sensitive downstream applications including PCR and qPCR.

Background

A sputum specimen is the name given to the mucus which is expectorated from the lower airways. High quality sputum samples should contain very little saliva, as this may contaminate the sputum sample with oral bacteria. Sputum samples are typically evaluated to look for infections such as Moraxella catarrhalis,Mycobacterium tuberculosisStreptococcus pneumoniae and Haemophilus influenza. Other pathogens can be detected in sputum as well, including HIV. In addition, sputum samples are useful in the detection of lung cancer or to evaluate chronic inflammation.

Kit Specifications
Time to Complete DNA Isolation
< 1 hour
Average Yield of DNA from 1.0 mL*
75 μg
Average Purity (OD260/280)
1.8 - 2.1

* Average DNA yield will vary depending on the health status of the donor

Storage Conditions and Product Stability 
This kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at 4°C upon arrival (stable for 2 years) or at -20oC for long term storage. All other kit components should be kept tightly sealed and stored at room temperature (15 – 25 °C) for up to 3 years without any reduction in kit performance.

 

 

Kit Components
Component Cat. 46200 (25 preps)
Slurry D 55 mL
Proteinase K 0.6 mL
Solution WN 4 mL
Wash Solution BE 9 mL
Elution Buffer B 8 mL
Mini Filter Spin Columns 25
Collection Tubes 25
Elution Tubes (1.7 mL) 25
Product Insert 1
Title Digital PCR to Detect and Quantify Heteroresistance in Drug Resistant Mycobacterium tuberculosis.
Journal PLoS One2013.
Authors Pholwat S, Stroup S, Foongladda S, Houpt E.