Soil Total RNA Purification Kit

For the rapid preparation of inhibitor-free total RNA from soil

  • Isolate high quality total RNA from a variety of soil samples
  • Process all types of soil, including common soil, compost and manure
  • Remove all traces of humic acids and other inhibitors of PCR
  • Isolates all sizes of RNA, including microRNA, without phenol
  • Complete kit including bead tubes and humic acid removal columns (HAR)
Items Cat. # Size
Soil RNA Purification Kit 27750 50 preps


Soil Total RNA Purification Kit

This kit provides a convenient and rapid spin column method to purify total RNA from small amounts of soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acids and other inhibitors of PCR using humic acid removal (HAR) columns and the organic substance removal solution (OSR).

Norgen’s Soil Total RNA Purification Kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA without the need for phenol. The purified RNA is of the highest integrity and is inhibitor-free for any downstream application including microbiome studies, real time PCR and reverse transcription PCR for gene expression analysis, Next Generation Sequencing and more.

 

 

Kit Specifications
Suggested Soil Input
(Clay, loam, sand, feces, compost)
500 mg
Type of Soil Processed All types, including common soil,
compost and manure
Maximum Column Binding Capacity 50 μg
Maximum Column Loading Volume 650 μL
Time to Complete Purifications 30 minutes

Storage Conditions and Product Stability 
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

 

 

Kit Components
Component Cat. 27750 (50 preps)
Lysis Buffer I 2 x 20 mL
Binding Buffer E 6 mL
Solution BX 9 mL
Wash Solution A 18 mL
Elution Solution A 6 mL
Bead Tubes 50
Spin Columns 50
Humic Acid Removal Columns 50
Collection Tubes 100
Elution Tubes (1.7 mL) 50
Product Insert 1

 

Title Microbiota and Metabolome Associated with Immunoglobulin A Nephropathy (IgAN).
Journal Microbiota and Metabolome of IgA Nephropathy Patients2014.
Authors Maria De Angelis, Eustacchio Montemurno, Maria Piccolo, Lucia Vannini, Gabriella Lauriero, Valentina Maranzano, Giorgia Gozzi, Diana Serrazanetti, Giuseppe Dalfino, Marco Gobbetti, Loreto Gesualdo.
Title Extraction of Bacterial RNA from Soil: Challenges and Solutions.
Journal Microbes and the Environment / JSME2012.
Authors Wang Y, Hayatsu M, Fujii T.
Title Detection of viable Cryptosporidium parvum in soil by reverse transcription real time PCR targeting hsp70 mRNA.
Journal Applied and Environmental Biology2011.
Authors Liang Z, Keeley A.