Soil DNA Isolation Kit (Magnetic Bead System)

  • Fast and easy processing using a magnetic bead system
  • Robust lysis system (chemical lysis combined with a mechanical homogenization)
  • Isolate high quality genomic DNA
  • High yields - Consistent, high yields of inhibitor-free DNA up to 50 kb plus
  • Isolate sequencing quality total DNA from a variety of microorganisms including bacteria, fungi and algae

 

Figure 1. DNA isolated from high humic acid (top soil) and regular soil (clay)

Items Cat. # Size
Soil DNA Isolation Kit (Magnetic Bead System) 58100 50 preps

Soil DNA Isolation Kit (Magnetic Bead System)

Norgen’s Soil DNA Isolation Kit (Magnetic Bead System) provides a fast and reproducible method for isolating genomic DNA from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid using the provided the OSR (Organic Substance Removal) Solution. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation.

Kit Specifications
Maximum Soil Input
0.25 g
Type of Soil Processed
All types of soil
Average Yield from 0.25 mL of Soil*
1 - 5 μg
Average Purity (OD260/280)
1.7 - 1.8
Time to Complete 12 Purifications
40 minutes

* Average DNA yield will vary depending on the donor

Storage Conditions and Product Stability 
All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). These reagents should remain stable for at least 2 years in their unopened containers.

 

 

Kit Components
Component Cat. 58100 (50 preps)
Lysis Buffer G 100 mL
Lysis Additive A 6 mL
Binding Buffer I 7 mL
OSR Solution 3 mL
Binding Buffer B 2 x 12 mL
Magnetic Bead Suspension 2 x 1.1 mL
Buffer SK 30 mL
Elution Buffer B 8 mL
Elution Tubes (1.7 mL) 50
Product Insert 1