Soil DNA Isolation Kit

For the rapid preparation of inhibitor-free DNA from all types of soil, including difficult samples with high humic acid content

  • Isolate total DNA from all microorganisms found in soil, including bacteria, fungi and algae
  • Process all types of soil, including common soil samples such as clay, loam and sandy soils and difficult samples with high humic acid such as compost, peat and manure
  • Remove all humic acids using the provided Humic Acid Removal Columns and the OSR Solution
  • Isolated DNA is free of inhibitors such as humic acid - ready for PCR applications and any metagenomic study
  • Easy- to-use beads for extraction - fast without any time consuming sample grinding
Figure 1.  Superior Removal of Humic Acid
Superior Removal of Humic Acid
Items Cat. # Size
Soil DNA Isolation Kit 26500 50 preps

Soil DNA Isolation Kit

This kit provides a convenient and rapid method for the isolation of DNA from microorganisms from soil samples. All types of soil samples can be processed with this kit, including common soil samples such as clay, loam and sandy soil and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid using the provided Humic Acid Removal Column and the OSR (Organic Substance Removal) Solution. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. DNA is of the highest quality and is fully compatible with downstream PCR and qPCR applications, any metagenomic study and more, as all humic acid substances and PCR inhibitors are removed during the isolation.



Figure 3.  High Yields of DNA 
High Yields of DNA
 Figure 4.  High Quality DNA free from Inhibitors Figure 5. Hierarchical Clustering Dendrogram. Figure 6. Principal Coordinate Analysis (PCoA).
High Quality DNA free from           Hierarchical Clustering Dendrogram.                                                 Principal Coordinate Analysis (PCoA).


Kit Specifications
Maximum Soil Input
250 mg
Type of Soil Processed
All soil types, including clay,
loam and sandy soil, compost
and manure
Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Time to Complete 10 Purifications
30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.



Kit Components
Component Cat. 26500 (50 preps)
Lysis Buffer G 45 mL
Lysis Additive A 6 mL
Binding Buffer I 7 mL
OSR Solution 3 mL
Buffer SK 60 mL
Wash Solution A 18 mL
Elution Buffer B 8 mL
Bead Tubes 50
Humic Acid Removal Columns 50
Spin Columns 50
Collection Tubes 100
Elution Tubes (1.7 mL) 50
Product Insert 1
Title Comparison of Total DNA Extraction Methods for Microbial Community from Polluted Soil
Journal Conference paper. 2015.
Authors Ana-Maria Tanase, Ioana Mereuta Iulia Chiciudean, Robertina Ionescu, Ligia Milea, Călina Petruța Cornea, Tatiana Vassu, Ileana Stoica
Title Microbial community distribution and genetic analysis in a sludge active treatment for a complex industrial wastewater: a study using microbiological and molecular analysis and principal component analysis
Journal Annals of Microbiology. 2015.
Authors Giulio Moretti, Federica Matteucci, Claudia Ercole, Francesco Vegliò, Maddalena Del Gallo
Title Molecular Characterization and Potential of Bacterial Species Associated with Cassava Waste
Journal Nigerian Food Journal. 2014.
Authors A.I. Elijah, , O.O. Atanda, A.R. Popoola, S.V.A. Uzochukwu
Title Insertion/Deletion-Based Approach for the Detection of Escherichia coli O157:H7 in Freshwater Environments.
Journal American Chemical Society. 2014.
Authors Shirley Y. Wong, Athanasios Paschos, Radhey S. Gupta, and Herb E. Schellhorn.
Title Network topology reveals high connectance levels and few key microbial genera within soils.
Journal Frontiers in Environmental Science. 2014.
Authors Lupatini M, Suleiman A, Jacques R, Antoniolli Z, Ferreira A, Kuramae E and Roesch L.
Title Optimization and Validation of Molecular Assays for Invasive Tunicate Monitoring in Environmental Water Samples.
Journal Macrothink Institute2013.
Authors Stewart-Clark S, Davidson J, Greenwood S.
Title Bioremediation of high organic load lagoon sediments: Compost addition and priming effects.
Journal Chemosphere2012.
Authors d'Errico G, Giovannelli D, Montano C, Milanovic V, Ciani M, Manini E.
Title Molecular Approaches to the Study of Biological Phosphorus Cycling.
Journal Soil Biology2010.
Authors Wasaki J. and Maruyama H.