RNA-Protein Purification Plus Kit

For sequential isolation of total RNA and total proteins from the same sample

  • Sequentially purify total RNA and total proteins from a single sample
  • Kit includes a gDNA elimination column
  • No sample splitting required
  • No phenol step required for efficient isolation
  • Ideal for small or difficult to obtain samples
  • Purify RNA and proteins from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
  • Rapid and efficient spin column procedure
Items Cat. # Size
RNA/Protein Purification Plus Kit 48200 50 preps

RNA/Protein Purification Plus Kit

This kit provides a rapid, single column method for the isolation and purification of total RNA (including miRNA) and proteins sequentially from a single sample of cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants. The total RNA and proteins are both column purified in under 25 minutes using a single column.

Purified RNA is of a high quality and yield, and is suitable for NGS, RT-qPCR and microarrays.

This kit eliminates DNA efficiently using a gDNA removal column.

Proteins are eluted in buffer and are ready for downstream applications such as Western Blots, Mass Spec and some ELISA applications. The proteins will not require precipitation, resuspending of pellets, or any further cleaning.

This kit is ideal for researchers who are interested in studying the transcriptome and proteome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/Protein Purification Plus Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNAand proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.

Protocol

Workflow

 

 

Kit Specifications
Column Binding Capacity (RNA) 50 μg
Column Binding Capacity (Protein) 200 μg
Maximum Column Loading Volume 650 μL
Size of RNA Purified All sizes
Average Yields:
HEK 293 Cells (1 x 106 cells)
HEK 293 Cells (1 x 106 cells)
Liver (15 mg)
Liver (15 mg)
10-15 μg
70-100 μg protein
30-35 μg RNA
100-150 μg protein

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT).

 

 

Kit Components
Component Cat. 48200 (50 preps)
Lysis Buffer Q 40 mL
Wash Solution A 38 mL
Elution Solution A 6 mL
Wash Solution C 30 mL
Binding Buffer A 8 mL
Elution Buffer C 4 mL
Protein Neutralizer 4 mL
Protein Loading Dye 2 mL
gDNA Removal Columns 50
RNA/Protein Purification Columns 50
Collection Tubes 150
Elution Tubes (1.7 mL) 100
Product Insert 1

 

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Title The binding activity of Mel-18 at the Il17a promoter is regulated by the integrated signals of the TCR and polarizing cytokines.
Journal European Journal of Immunology. 2011.
Authors Hod-Dvorai R, Jacob E, Boyko Y, Avni O.
Title Proteomics Analysis of Human Optic Nerve Head Astrocytes Following Biomechanical Strain.
Journal Molecular and Cellular Proteonomics2011.
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Title PU.1 is a major transcriptional activator of the tumour suppressor gene LIMD1.
Journal FEBS Letters. 2011.
Authors Foxler DE, James V, Shelton SJ, Vallim TQ, Shaw PE, Sharp TV.
Title The calcium-sensing receptor in taste tissue
Journal Biochemical and Biophysical Research Communications2009.
Authors San Gabriel A, Uneyama H, Maekawa T, Torii K.