This kit provides a rapid method for the isolation and purification of total RNA and DNA sequentially from a single sample of cultured animal cells of less than 500 tissues, blood, bacteria, yeast, fungi. The lysate is passed over two columns: 1) DNA column and 2) an RNA column. The purified RNA includes all RNA sizes including microRNA. Both DNA and RNA are of the highest purity and yield and are eluted in as little as 20 μL.
The RNA/DNA Purification Micro Kit is ideal for researchers who are interested in studying the genome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen's RNA/DNA Purification Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA and DNA are derived from the same sample, thereby eliminating inconsistent results.The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.