RNA/DNA Purification Micro Kit

For sequential isolation of total RNA and genomic DNA from the same sample

  • Sequentially isolate and purify total RNA and DNA from a single sample
  • Two specialized small diameter columns: 1) for DNA and 2) for RNA
  • The RNA column is for the purification of Total RNA including microRNA
  • ​Ideal for cell number inputs of 500,000 and as little as 5 cells
  • Elute DNA or RNA in as little as 20 μL for clean and concentrated sample
  • No need to split the lysate, or to use phenol or precipitation methods
  • Rapid and efficient spin column procedure - it takes only 30 minutes
Items Cat. # Size
RNA/DNA Purification Micro Kit 50300 50 preps

 


RNA/DNA Purification Micro Kit

This kit provides a rapid method for the isolation and purification of total RNA and DNA sequentially from a single sample of cultured animal cells of less than 500 tissues, blood, bacteria, yeast, fungi. The lysate is passed over two columns: 1) DNA column and 2) an RNA column. The purified RNA includes all RNA sizes including microRNA. Both DNA and RNA are of the highest purity and yield and are eluted in as little as 20 μL.

The RNA/DNA Purification Micro Kit is ideal for researchers who are interested in studying the genome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen's RNA/DNA Purification Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA and DNA are derived from the same sample, thereby eliminating inconsistent results.The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.

Protocol

Workflow

 

 

Kit Specifications
Maximum Column Binding Capacity
35 μg for RNA
10 μg for DNA
Maximum Column Loading Volume
650 μL
Elution Volume
20-50 μL
Size of RNA Purified
All sizes, including
small RNA (< 200 nt)
Time to Complete 10 Purifications
30 minutes
Maximum Amount of Starting Material:
Animal Cells
Animal Tissues
Blood
 
5 x 105 cells
5 mg (for selected tissues)
50 µL
Average Yields*
HeLa Cells (5 x 105 cells)
HeLa Cells (5 x 105 cells)
RNA: 10-12 μg
DNA: 1-2 μg

Storage Conditions and Product Stability 
Store Proteinase K at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers

 

 

Kit Components
Component Cat. 50300 (50 preps)
Lysis Buffer Q 40 mL
Wash Solution A 2 x 38 mL, 1 x 18 mL
Elution Solution A 6 mL
Elution Buffer F 6 mL
gDNA Purification Micro Columns 50
RNA Purification Micro Columns 50
Collection Tubes 100
Elution Tubes (1.7 mL) 100
Product Insert 1