RNA/DNA Purification Kit

For sequential isolation of total RNA and genomic DNA from the same sample

  • Sequentially isolate and purify total RNA and DNA from a single sample
  • Two column system: one for DNA and one for RNA
  • The RNA column is for the purification of total RNA including microRNA
  • No need to split the lysate, or to use phenol or precipitation methods
  • Rapid and efficient spin column procedure - it takes only 30 minutes
Items Cat. # Size
RNA/DNA Purification Kit 48700 50 preps


RNA/DNA Purification Kit

The kit provides a rapid method for the isolation and purification of total RNA and DNA sequentially from a single sample of cultured animal cells and tissues, blood, bacteria, yeast, or fungi. The lysate is passed over two columns: 1) a DNA column and 2) an RNA column. Total RNA of all sizes is purified, including microRNA. Both DNA and RNA are of the highest purity and yield.

This kit is ideal for researchers who are interested in studying the genome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/DNA Purification Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA and DNA are derived from the same sample, thereby eliminating inconsistent results.The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.

Protocol

Workflow

 

 

Kit Specifications
Maximum Binding Capacity
50 μg for RNA
20 μg for DNA
Maximum Column Loading Volume
650 μL
Size of RNA Purified
All sizes, including small
RNA (< 200 nt)
Maximum Amount of Starting Material:
Animal Cells
Animal Tissues
Blood
Bacteria
Yeast
Fungi
Plant Tissues
5 x 106 cells
25 mg (for most tissues)
200 μL
1 x 109 cells
1 x 108 cells
50 mg
50 mg
Time to Complete 10 Purifications
30 minutes
RNA Yield
HEK 293 Cells (1 x 106 cells)
HEK 293 Cells (1 x 106 cells)
Liver (15 mg)
Liver (15 mg)
10-15 μg RNA
2-4 μg DNA
30-35 μg RNA
4-6 μg DNA

Storage Conditions and Product Stability 
Store Proteinase K at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

 

 

Kit Components
Component Cat. 48700 (50 preps)
Lysis Buffer Q 40 mL
Wash Solution A 2 x 38 mL, 1 x 18 mL
Elution Solution A 6 mL
Elution Buffer F 15 mL
gDNA Purification Columns 50
RNA Purification Columns 50
Collection Tubes 100
Elution Tubes (1.7 mL) 100
Product Insert 1

 

Title 5-HT1A receptors on mature dentate gyrus granule cells are critical for the antidepressant response
Journal Nature Neuroscience. 2015.
Authors Samuels BA, Anacker C, Hu A, Levinstein MR, Pickenhagen A, Tsetsenis T, Madroñal N, Donaldson ZR, Drew LJ, Dranovsky A, Gross CT, Tanaka KF, Hen R
Title Total Exposure Study Analysis consortium: a cross-sectional study of tobacco exposures
Journal BMC Public Health. 2015.
Authors Andrew W. Bergen, Ruth Krasnow, Harold S. Javitz, Gary E. Swan, Ming D. Li, James W. Baurley, Xiangning Chen, Lenn Murrelle and Barbara Zedler
Title MET dysregulation is a hallmark of aggressive disease in multiple myeloma patients.
Journal British Journal of Haematology. 2014.
Authors Rocci A, Gambella M, Aschero S, Baldi I, Trusolino L, Cavallo F, Gay F, Larocca A, Magarotto V, Omedè P, Isaia G, Bertotti A, Liberati AM, Catalano L, De Rosa L, Musto P, Vallone R, Falcone A, Drandi D, Ladetto M, Comoglio PM, Boccadoro M, Palumbo A.
Title High XBP1 expression is a marker of better outcome in multiple myeloma patients treated with bortezomib.
Journal Haematologica. 2014.
Authors Gambella M, Rocci A, Passera R, Gay F, Omedè P, Crippa C, Corradini P, Romano A, Rossi D, Ladetto M, Boccadoro M, Palumbo A
Title The Role of Urinary Cathelicidin (LL-37) and Human β-defensin 1 (hBD-1) in Uncomplicated Escherichia coli Urinary Tract Infections.
Journal Infection and Immunity.. 2014.
Authors Nielsen KL, Dynesen P, Larsen P, Jakobsen L, Andersen PS, Frimodt-Møller N.
Title Human RNA Methyltransferase BCDIN3D Regulates MicroRNA Processing.
Journal Cell. 2012
Authors Xhemalce B, Robson SC, Kouzarides T.
Title Coccidioidomycosis: ADA levels, serologic parameters, culture results, and PCR testing in pleural fluid.
Journal Chest Journal. 2012
Authors Thompson B, Sharma S, Bays D, Pruitt R; Engelthaler D; Bowers J; Driebe E; Davis M, Libke R, Cohen S; Pappagianis D.
Title Microarray Analysis of Rat Sensory Ganglia after Local Inflammation Implicates Novel Cytokines in Pain
Journal PLOS One. 2012
Authors Strong J, Xie W, Coyle D, Zhang J-M.