RNA Clean-Up and Concentration Kit

For rapid and efficient clean-up and concentration of total RNA, including microRNA, without phenol

  • Clean-up & concentrate total RNA (including miRNA) in minutes
  • Clean-up & concentrate RNA from TRIzol®, TRI Reagent®, etc
  • Clean-up RNA from contaminants including enzymes, primers, nucleotides
  • Rapid spin-column protocol, elute in 20 µL
  • 96-well format for high throughput processing & small spin column formats for 8 µL are available
  • Purified RNA is fully compatible with all downstream applications
Items Cat. # Size
RNA Clean-Up and Concentration Kit 23600 50 preps
RNA Clean-Up and Concentration Kit 43200 100 Preps
RNA Clean-Up and Concentration 96-Well Kit 25100 2 x 96-well plates

 

RNA Clean-Up and Concentration Kit

This kit is designed to clean-up and concentrate RNA (including miRNA) from TRIzol® and TRI Reagent®, enzymatic reactions, in vitro transcription, labelling reactions, etc. This is a robust kit for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications.

The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.

The kit elutes RNA in 20 µL for all types of downstream applications. For smaller elution volumes of 8 µL, please refer to the microspin column kits such as theRNA Clean-Up and Concentration Micro-Elute™ Kit for the highest concentration of RNA sample.

 

 


RNA Clean-Up and Concentration Kit

This kit is designed to clean-up and concentrate RNA (including miRNA) from TRIzol® and TRI Reagent®, enzymatic reactions, in vitro transcription, labelling reactions, etc. This is a robust kit for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications.

The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.

The kit elutes RNA in 20 µL for all types of downstream applications. For smaller elution volumes of 8 µL, please refer to the microspin column kits such as theRNA Clean-Up and Concentration Micro-Elute™ Kit for the highest concentration of RNA sample.

 

 

Title Synthetically modified mRNA for efficient and fast human iPS cell generation and direct transdifferentiation to myoblasts
Journal Biochemical and Biophysical Research Communications. 2015.
Authors David Preskey, Thomas F. Allison, Mark Jones, Kamel Mamchaou, and Christian Unger
Title A differentially expressed set of micrornas in cerebro-spinal fluid (CSF) can diagnose CNS malignancies
Journal Oncotarget. 2015.
Authors Alessandra Drusco, Arianna Bottoni, Alessandro Lagana, Mario Acunzo, Matteo Fassan, Luciano Cascione, Anna Antenucci, Prasanthi Kumchala, Caterina Vicentini, Marina P Gardinam, Hansjuerg Alder, Mariantonia A Carosi, Mario Ammirati, Stefano Gherardi, Marilena Luscri, Carmine Carapella, Nicola Zanesi, Carlo M Croce
Title Blocking of targeted microRNAs from next-generation sequencing libraries
Journal Nucleic Acids Research. 2015.
Authors Brian S. Roberts, Andrew A. Hardigan, Marie K. Kirby, Meredith B. Fitz-Gerald, C. Mel Wilcox, Robert P. Kimberly and Richard M. Myers
Title Expression of miRNAs in Bull Spermatozoa Correlates with Fertility Rates.
Journal Reproduction in Domestic Animals. 2015.
Authors Fagerlind M, Stålhammar H, Olsson B, Klinga-Levan K
Title The Streptococcus pyogenes Orphan Protein Tyrosine Phosphatase, SP-PTP, Possesses Dual Specificity, and Essential Virulence Regulatory Functions
Journal Molecular Microbiology. 2015.
Authors Sashi Kant, Shivani Agarwal, Preeti Pancholi and Vijay Pancholi
Title Tracking the Elusive Function of Bacillus subtilis Hfq
Journal PLOS ONE. 2015.
Authors Tatiana Rochat, Olivier Delumeau, Nara Figueroa-Bossi, Philippe Noirot, Lionello Bossi, Etienne Dervyn, Philippe Bouloc
Title A Multi-Omics Approach Identifies Key Hubs Associated with Cell Type-Specific Responses of Airway Epithelial Cells to Staphylococcal Alpha-Toxin
Journal PLOS ONE2015.
Authors Erik Richter, Manuela Harms, Katharina Ventz, Philipp Gierok, Ravi Kumar Chilukoti, Jan-Peter Hildebrandt, Jörg Mostertz, Falko Hochgräfe
Title Atrial fibrillation and rapid acute pacing regulate adipocyte/adipositas-related gene expression in the atria
Journal International Journal of Cardiology2015.
Authors R.K. Chilukoti, A. Giese, W. Malenke, G. Homuth, A. Bukowska, A., Goette, S.B. Felix, J. Kanaan, H.-G. Wollert, K. Evert, S. Verheule, P. Jais, S.N. Hatem, U. Lendeckel, C. Wolke
Title The elucidation of stress memory inheritance in Brassica rapa plants
Journal Frontiers in Plant Science2015.
Authors Andriy Bilichak, Yaroslav Ilnytskyy, Rafal Wóycicki, Nina Kepeshchuk, Dawson Fogen and Igor Kovalchuk
Title LARP1 post-transcriptionally regulates mTOR and contributes to cancer progression
Journal Oncogene2014.
Authors M Mura, T G Hopkins, T Michael, N Abd-Latip, J Weir, E Aboagye, F Mauri, C Jameson, J Sturge, H Gabra, M Bushell, A E Willis, E Curry and S P Blagden
Title Adipocyte-derived exosomal miRNAs: a novel mechanism for obesity-related disease
Journal Pediatric Research2014.
Authors Sarah C. Ferrante, Evan P. Nadler, Dinesh K. Pillai, Monica J. Hubal, Zuyi Wang, Justin M. Wang, Heather Gordish-Dressman, Emily Koeck, Samantha Sevilla, Andrew A. Wiles and Robert J. Freishtat
Title Impact of priming on the response of neutrophils to human neutrophil alloantigen-3a antibodies.
Journal Transfusion2014.
Authors Berthold T, Muschter S, Schubert N, Wesche J, Ameling S, Teumer A, Reil A, Bux J, Bakchoul T, Greinacher A.
Title RNA at 92 °C: the non-coding transcriptome of the hyperthermophilic archaeon Pyrococcus abyssi.
Journal RNA Biology2013.
Authors Toffano-Nioche C, Ott A, Crozat E, Nguyen AN, Zytnicki M, Leclerc F, Forterre P, Bouloc P, Gautheret D.
Title Definition of the σW Regulon of Bacillus subtilis in the Absence of Stress.
Journal PLOS One2012.
Authors Zweers JC, Nicolas P, Wiegert T, van Dijl JM, Denham EL.
Title Dronedarone prevents microcirculatory abnormalities in the left ventricle during atrial tachypacing.
Journal British Journal of Pharmacology2011.
Authors Bukowska A, Hammw?hner M, Sixdorf A, Schild L, Wiswedel I, R?hl FW, Wolke C, Lendeckel U, Aderkast C, Bochmann S, Chilukoti RK, Mostertz J, Bramlage P, Goette A.
Title Transcriptional analysis of molecular interactions between Pseudomonas fluorescens strain TDK1, Oryza sativa and Cnaphalocrocis medinalis
Journal Journal of Applied Entomology2010.
Authors K. Saveetha, L. Karthiba, M. Raveendran, D. Saravanakumar, T. Raguchander and R. Samiyappan
Title Shedding light on an extremophile lifestyle through transcriptomics
Journal New Phytologist2009.
Authors M. Dassanayake, J. S. Haas, H. J. Bohnert and J. M. Cheeseman
Title Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032
Journal PLoS ONE2007.
Authors Gioia J, Yerrapragada S, Qin X, Jiang H, Igboeli OC, Muzny D, Dugan-Rocha S, Ding Y, Hawes A, Liu W, Perez L, Kovar C, Dinh H, Lee S, Nazareth L, Blyth P, Holder M, Buhay C, Tirumalai MR, Liu Y, Dasgupta I, Bokhetache L, Fujita M, Karouia F, Eswara Moorthy P, Siefert J, Uzman A, Buzumbo P, Verma A, Zwiya H, McWilliams BD, Olowu A, Clinkenbeard KD, Newcombe D, Golebiewski L, Petrosino JF, Nicholson WL, Fox GE, Venkateswaran K, Highlander SK, Weinstock GM.
Title Analysis of Human TAAR8 and Murine Taar8b Mediated Signaling Pathways and Expression Profile.
Journal International Journal of Molecular Sciences. 2014.
Authors Jessica Mühlhaus, Juliane Dinter, Daniela Nürnberg, Maren Rehders, Maren Depke, Janine Golchert, Georg Homuth, Chun-Xia Yi, Silke Morin, Josef Köhrle, Klaudia Brix, Matthias Tschöp, Gunnar Kleinau and Heike Biebermann.
Title Alterations in Insulin-Signaling and Coagulation Pathways in Platelets during Hyperglycemia-Hyperinsulinemia in Healthy Non-Diabetic Subject.
Journal JThrombosis Research. 2014.
Authors A. Koneti Rao, Robert J. Freishtat, Gauthami Jalagadugula, Anamika Singh, Guangfen Mao, Andrew Wiles, Peter Cheung, Guenther Boden.
Title Rapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP® using reverse transcription-recombinase polymerase amplification.
Journal Journal of Virology Methods. 2014.
Authors Shulu Zhang, Michel Ravelonandro, Paul Russell, Nathan McOwen, Pascal Briard, Seven Bohannon, Albert Vrient.
Title Bacillus pumilus Reveals a Remarkably High Resistance to Hydrogen Peroxide Provoked Oxidative Stress.
Journal PLoS One. 2014.
Authors Handtke S, Schroeter R, Jürgen B, Methling K, Schlüter R, Albrecht D, Van Hijum S, Bongaerts J, Maurer K, Lalk M, Schweder T, Hecker M, Voigt B
Title Activation of the alternative sigma factor SigB of Staphylococcus aureus following internalization by epithelial cells - An in vivo proteomics perspective.
Journal International Journal of Medical Microbiology. 2013.
Authors Pförtnera H, Buriana M, Michalik S, Depke M, Hildebrandt P, Dhople V, Pané-Farré J, Hecker M, Schmidt F, Völker U
Title Interactions between Blood-Borne Streptococcus pneumoniae and the Blood-Brain Barrier Preceding Meningitis.
Journal PLoS One. 2013.
Authors Iovino F, Orihuela CJ, Moorlag HE, Molema G, Bijlsma JJ.
Title Metabolic engineering of Bacillus subtilis for growth on overflow metabolites.
Journal Microbial Cell Factories. 2013.
Authors Kabisch J, Pratzka I, Meyer H, Albrecht D, Lalk M, Ehrenreich A, Schweder T.
Title Ocular fibroblast types differ in their mRNA profiles—implications for fibrosis prevention after aqueous shunt implantation.
Journal Comparative Biochemistry and Physiology. 2013.
Authors Löbler M, Buß D, Kastner C, Mostertz J, Homuth G, Ernst M, Guthoff R, Wree A, Stahnke T, Fuellen G, Voelker U, Schmitz P.
Title Effects of irbesartan on gene expression revealed by transcriptome analysis of left atrial tissue in a porcine model of acute rapid pacing in vivo.
Journal International Journal of Cardiology. 2013.
Authors Chilukoti RK, Mostertz J, Bukowska A, Aderkast C, Felix SB, Busch M, Völker U, Goette A, Wolke C, Homuth G, Lendeckel U.
Title Circulating microRNA expression profiles associated with systemic lupus erythematosus.
Journal Arthritis and Rheumatism. 2013.
Authors Carlsen AL, Schetter AJ, Nielsen CT, Lood C, Knudsen S, Voss A, Harris CC, Hellmark T, Segelmark M, Jacobsen S, Bengtsson AA, Heegaard NH.
Title Identification of Critical Amino Acids within the Nucleoprotein of Tacaribe Virus Important for Anti-Interferon Activity.
Journal The Journal of Biological Chemistry. 2013.
Authors Harmon B, Kozina C, Maar D, Carpenter TS, Branda CS, Negrete OA, Carson BD.
Title Ecological functions and differentially expressed transcripts of translucent bracts in an alpine 'glasshouse' plant Rheum nobile (Polygonaceae).
Journal Planta. 2012.
Authors Zhang D, Liu B, Zhao C, Lu X, Wan D, Ma F, Chen L, Liu J.
Title Dronedarone prevents microcirculatory abnormalities in the left ventricle during atrial tachypacing in pigs.
Journal British Journal of Pharmacology. 2012.
Authors Bukowska A, Hammwöhner M, Sixdorf A, Schild L, Wiswedel I, Röhl FW, Wolke C, Lendeckel U, Aderkast C, Bochmann S, Chilukoti RK, Mostertz J, Bramlage P, Goette A.
Title Temporal Profiling of Gene Networks Associated with the Late Phase of Long-Term Potentiation In Vivo.
Journal PLoS ONE. 2012.
Authors Ryan MM, Ryan B, Kyrke-Smith M, Logan B, Tate WP, Abraham W, Williams J.
Title Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Journal Proceedings of the National Academy of Sciences of the United States of America. 2012.
Authors Elsholz AK, Turgay K, Michalik S, Hessling B, Gronau K, Oertel D, Mäder U, Bernhardt J, Becher D, Hecker M, Gerth U.
Title Incorporation of Transmembrane Hydrophobic Mutations in the TCR Enhance Its Surface Expression and T Cell Functional Avidity.
Journal The Journal of Immunology. 2012.
Authors Haga-Friedman A, Horovitz-Fried M, J Cohen C.
Title Primary cultures of glomerular parietal epithelial cells or podocytes with proven origin.
Journal PLoS One. 2012.
Authors Kabgani N, Grigoleit T, Schulte K, Sechi A, Sauer-Lehnen S, Tag C, Boor P, Kuppe C, Warsow G, Schordan S, Mostertz J, Chilukoti RK, Homuth G, Endlich N, Tacke F, Weiskirchen R, Fuellen G, Endlich K, Floege J, Smeets B, Moeller MJ.
Title Promiscuous targeting of polytopic membrane proteins to SecYEG or YidC by the Escherichia coli signal recognition particle.
Journal Molecular Biology of the Cell. 2012.
Authors Welte T, Kudva R, Kuhn P, Sturm L, Braig D, Müller M, Warscheid B, Drepper F, Koch HG.
Title The alternative sigma factor B modulates virulence gene expression in a murine Staphylococcus aureus infection model but does not influence kidney gene expression pattern of the host.
Journal International Journal of Medicine Microbiology. 2011.
Authors Depke M, Burian M, Schäfer T, Bröker BM, Ohlsen K, Völker U.
Title Phenotype Enhancement Screen of a Regulatory spx Mutant Unveils a Role for the ytpQ Gene in the Control of Iron Homeostasis.
Journal PLoS One. 2011.
Authors Zuber P, Chauhan S, Pilaka P, Nakano M, Gurumoorthy S, Lin A, Barendt S, Chi B, Antelmann H and Mäder U.
Title RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y.
Journal Molecular Microbiology 2011.
Authors Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J.
Title S-bacillithiolation protects against hypochlorite stress in Bacillus subtilis as revealed by transcriptomics and redox proteomics.
Journal Molecular and Cellular Proteomics 2011.
Authors Chi BK, Gronau K, Maeder U, Hessling B, Becher D, Antelmann H.
Title Characterization of the global impact of low temperature gas plasma on vegetative microorganisms.
Journal Proteomics 2011.
Authors Winter T, Winter J, Polak M, Kusch K, Mäder U, Sietmann R, Ehlbeck J, Hijum S, Weltmann K, Hecker M, Kusch H.