ProteoSpin™ Total Protein Concentration and Detergent Clean-Up Maxi Kit

  1. Ana Sayfa
  2. Ürünler
  3. Protein İzolasyon Kitleri

For fast and simple removal of detergents from protein samples

  • Concentrate protein samples and remove detergents, salts and denaturants at the same time
  • Rapid and convenient spin-column protocol is completed in about 20 minutes
  • Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20
Items Cat. # Size
ProteoSpin™ Total Protein Concentration and Detergent Clean-Up Maxi Kit 17150 4 preps

ProteoSpin™ Total Protein Concentration and Detergent Clean-Up Maxi Kit

This kit simultaneously removes detergents, salts, denaturants and other contaminants from protein samples while concentrating and buffer exchanging with a rapid and convenient spin column method in about 40 minutes. All types of detergents including ionic, non-ionic and zwitterionic detergents can be effeciently removed. Both free detergents and those bound to proteins are effectively removed. Unlike other commercially available kits that can only handle one detergent and often do not remove denaturants, this kit removes a variety of detergents and denturants that may be used during the purification of the protein sample while providing high protein recovery.

No further cleaning of the protein sample is required and it can be used directly in a variety of downstream applications including mass spectrometry, SDS-PAGE, isoelectric focusing, and NMR spectroscopy.

Kit Specifications
Maximum Protein Input
8 mg
Minimum Protein Input
0.25 mg
Protein Recovery
Up to 100%
% Detergent Removal
> 95%
Minimum Elution Volume
4 mL
Time to Process 12 Samples
40 minutes

Storage Conditions
Unopened solutions should be stored at room temperature. Once opened, the solutions should be stored at 4°C when not in use, except for the Basic and Acidic Binding Buffers, which should be stored at room temperature.

 

 

 

Kit Components
Component Cat. 17150 (4 preps)
Binding Buffer A 20 mL
Binding Buffer N 20 mL
Wash Solution C 60 mL
Wash Solution CIP 60 mL
Wash Solution N 60 mL
Wash Solution NIP 60 mL
Elution Buffer C 2 x 30 mL
Protein Neutralizer 2 x 4 mL
Maxi Spin Columns (assembled with collection tubes) 4
Elution Tubes 4
Product Insert 1
Title An Optimized Approach for Protein Residue Extraction and Identification from Ceramics After Cooking.
Journal Journal of Archaeological Method and Theory. 2011.
Authors Barker A, Venables B, Stevens S, Seeley K, Wang P and Wolverton S
Title Two-dimensional electrophoresis and IgE-mediated food allergy.
Journal Electrophoresis. 2010.
Authors De Angelis M, Di Cagno R, Minervini F, Rizzello CG, Gobbetti M.
Title Proteome of Human Calcium Kidney Stones.
Journal Urology. 2010.
Authors Canales BK, Anderson L, Higgins L, Ensrud-Bowlin K, Roberts KP, Wu B, Kim IW, Monga M.
Title Structural stability of Amandin, a major allergen from almond (Prunus dulcis), and its acidic and basic polypeptides.
Journal Journal of Agriculture and Food Chemistry. 2009.
Authors Albillos SM, Menhart N, Fu TJ.
Title Comprehensive Proteomic Analysis of Human Calcium Oxalate Monohydrate Kidney Stone Matrix
Journal Journal of Endourology. 2008.
Authors Benjamin K. Canales, Lorraine Anderson, Leeann Higgins, Joel Slaton, Ken P. Roberts, Nathan Liu, Manoj Monga.
Title Classification of Calcium Oxalate, Phosphate and Brushite Kidney Stones by Protein Content.
Journal AMSM Poster. 2007.
Authors LeeAnn Higgins; Benjamin K. Canales; Lorraine B. Anderson; Joel W. Slaton; Nathan W. Liu; Ken P. Roberts; Manoj Monga.
Title A method for the selective isolation and enrichment of carrier protein-bound low-molecular weight proteins and peptides in the blood.
Journal Proteomics Clinical Applications. 2007.
Authors Serena Camerini, Maria Letizia Polci, Lance A. Liotta, Emanuel F. Petricoin, Weidong Zhou.
Title Identification of eggshell membrane proteins and purification of ovotransferrin and beta-NAGase from hen egg white.
Journal Protein Journal. 2006.
Authors Ahlborn GJ, Clare DA, Sheldon BW, Kelly RW.