Plasmid MiniPrep DNA Kit

For rapid and convenient plasmid DNA preparations

  • High yield of plasmid DNA
  • Purify plasmids up to 13 kb
  • Plasmid DNA is ready for restriction digestion, bacterial transformation, sequencing and more
  • Rapid spin-column format and 96-well format available
Items Cat. # Size
Plasmid DNA MiniPrep Kit 13300 50 preps
Plasmid DNA MiniPrep Kit 46400 250 preps
Plasmid DNA MiniPrep 96-well Kit 25900 1 x 96-well plate
Plasmid DNA MiniPrep 96-well Kit 43300 4 x 96-well plates

Plasmid MiniPrep DNA Kit

This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.

Kit Specifications
Column Binding Capacity
25 µg
Size of Plasmids Purified
Up to 13,000 bp
Average Yield from 1.5 mL of Culture
Up to 20 µg
Time to Complete 10 Purifications
30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. The RNAse should be stored at -20°C upon arrival. The Resuspension Buffer should be stored at 4°C upon addition of RNAse enzyme.

 

 

Kit Components
Component Cat. 13300 (50 preps) Cat. 46400 (250 preps)
Resuspension Solution AZ 12 mL 60 mL
Lysis Buffer N 40 mL 80 mL
Buffer TN 20 mL 130 mL
Wash Solution E 12 mL 2 x 18 mL
Elution Buffer K 8 mL 30 mL
RNase A 1 vial 1 vial
Spin Columns 50 250
Collection Tubes 50 250
Elution Tubes (1.7 mL) 50 250
Product Insert 1 1
     
Component Cat. 25900 (96-well)  
Resuspension Buffer 24 mL  
Lysis Solution 30 mL  
Binding Solution 40 mL  
Wash Solution 12 mL  
Elution Buffer 20 mL  
RNAse 1 vial  
96-Well Deep Plate 1  
96-Well Filter Plate 1  
96-Well Plate 1  
Adhesive Tape 6  
96-Well Collection Plate 2  
96-Well Elution Plate 1  
Product Insert 1
Title Plasmid profile of Staphylococcus aureus from orthopaedic patients in Ahmadu Bello University Teaching Hospital Zaria, Nigeria
Journal African Journal of Microbiology Research. 2015.
Authors Obajuluwa, A. F., Olayinka, B. O., Adeshina, G. O., Onaolapo, J. A. and Afolabi-Balogun, N. B.