Norgen's Plasma/Serum Circulating Nucleic Acid Purification Mini Kit provides a fast, reliable and simple procedure for isolating circulating nucleic acids (mRNA, miRNA, exosomal RNA, viral RNA, DNA and viral DNA) from various amounts of plasma/serum ranging from 0.25 mL to 2 mL.
Purification is based on the use of Norgen’s proprietary resin as the separation matrix. The kit is able to isolate all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA and circulating DNA. Norgen’s Plasma/Serum Circulating Nucleic Acid Purification Mini Kit provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating NA from large sample volumes. Free-circulating NA can be isolated from either fresh or frozen samples using this kit, and the kit allows for the concentration of NA that is present in low concentrations (1-100ng/mL in human plasma). Typical yields of free-circulating NA will vary depending on the input sample, as the amount of NA present in plasma and serum will depend upon the health status of the individual and the level of nucleases present in the blood. This kit is suitable for the purification of nucleic acids from serum or plasma prepared from blood collected on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
Free-circulating nucleic acids in plasma and serum have the potential to provide biomarkers for certain cancers and disease states, and include tumor-specific extracellular nucleic acid fragments and fetal DNA in maternal blood. Free-circulating DNA is usually present as short fragments of less than 1000 bp, whereas free-circulating RNA and exosomal RNA in plasma and serum are usually present as short fragments of less than 1000 nt. In addition, plasma or serum also contains free-circulating miRNA that are as small as 21 nt. Furthermore, circulating viral DNA or viral RNA may be present in the plasma or the serum of viral-infected patients. Norgen’s Plasma/Serum Circulating Nucleic Acid Purification Mini Kit provides an efficient method for the purification of all sizes of these fragmented free-circulating nucleic acid from human plasma or serum.