Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format)

For the rapid and simple isolation of circulating DNA from plasma/serum samples

  • Isolate all sizes of circulating DNA from plasma and serum samples
  • Isolate Viral and Bacterial DNA
  • Versatile plasma and serum input volumes (50 µL - 400 µL)
  • Concentrate circulating DNA into small elution volumes
  • Different elution strategies depending on the downstream application
  • Isolate inhibitor-free circulating DNA for any application including PCR, qPCR, methylation-sensitive PCR
  • Compatible with Streck Cell-Free DNA BCT® Tubes
Items Cat. # Size
Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format) 50600 50 preps

Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format) provides an efficient method for the purification of fragmented free-circulating DNA from 50 µL - 400 µL of human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit. The kit will also isolate viral and bacterial DNA from plasma/serum.

Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. Preparation time for a single sample is less than 30 minutes. The purified, inhibitor-free plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis. ​​

Kit Specifications
Minimum Plasma/Serum Input
50 μL
Maximum Plasma/Serum Input
400 μL
Minimum Elution Volume
100 μL
Time to Complete Purifications
< 30 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25oC) for up to 1 year without showing any reduction in performance.

 

 

Kit Components
Component Cat. 13300 (50 preps)
Lysis Buffer D 65 mL
Slurry A2 14 mL
Binding Buffer B 20 mL
Wash Solution A 2 x 38 mL
Elution Buffer B 8 mL
Mini Filter Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1
Title Comprehensive evaluation of methods to isolate, quantify, and characterize circulating cell-free DNA from small volumes of plasma
Journal Analytical and Bioanalytical Chemistry. 2015.
Authors Mauger F, Dulary C, Daviaud C, Deleuze JF, Tost J.
Title Cow’s Milk Contamination of Human Milk Purchased via the Internet
Journal Pediatrics2015.
Authors Sarah A. Keim, Manjusha M. Kulkarni, Kelly McNamara, Sheela R. Geraghty, Rachael M. Billock, Rachel Ronau, Joseph S. Hogan, Jesse J. Kwiek
Title Microbial contamination of human milk purchased via the Internet
Journal Pediatrics2013.
Authors Keim SA, Hogan JS, McNamara KA, Gudimetla V, Dillon CE, Kwiek JJ, Geraghty SR.