Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)

For the rapid and simple isolation of circulating DNA from plasma/serum samples

  • Isolate all sizes of circulating DNA from plasma and serum samples
  • Versatile plasma and serum input volumes (0.4 mL to 2 mL)
  • Concentrate circulating DNA into small elution volumes
  • Isolate inhibitor-free circulating DNA
  • Compatible with Streck Cell-Free DNA BCT® Tubes
Items Cat. # Size
Plasma/Serum Circulating DNA Isolation Midi Kit (Slurry Format) 51200 20 Preps

Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format) provides an efficient method for the purification of fragmented free-circulating DNA from 0.4 mL to 2 mL of human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit.

Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100 ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. Preparation time for a single sample is less than 45 minutes. The purified plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.
Kit Specifications
Minimum Plasma/Serum Input
400 μL
Maximum Plasma/Serum Input
2 mL
Minimum Elution Volume
100 μL
Time to Complete Purifications
< 45 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance.

 

 

Kit Components
Component Cat. 51200 (20 preps)
Lysis Buffer D 125 mL
Slurry A1 7 mL
Binding Buffer B 6 mL
Wash Solution A 2 x 38 mL
Binding Buffer C 6 mL
Elution Buffer B 8 mL
Mini Filter Spin Columns 20
Collection Tubes 40
Spin Columns 20
Elution tubes (1.7 mL) 40
Product Insert 1