Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format)

  • For the rapid and simple isolation of circulating DNA from plasma/serum samples

    • Isolate all sizes of circulating DNA from plasma and serum samples
    • Isolate viral and bacterial DNA
    • Versatile plasma and serum input volumes (2 to 10 mL)
    • Isolate inhibitor-free cell-free circulating DNA
    • Purify high-quality DNA in 15-20 minutes
    • Compatible with Streck Cell-Free DNA BCT® Tubes
Items Cat. # Size
Plasma/Serum Circulating DNA Isolation Maxi Kit (Slurry Format) 51300 10 Preps

Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format) provides an efficient method for the purification of fragmented free-circulating DNA from 2 mL to 10 mL of human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit.

Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100 ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. Preparation time for a single sample is less than 45 minutes. The purified plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.
Kit Specifications
Minimum Plasma/Serum Input
2 mL
Maximum Plasma/Serum Input
10 mL
Size of DNA Purified
≥ 50 bp
Elution Volume
100 μL
Time to Complete 10 Purifications
15-20 minutes
Average Yields
Variable depending on specimen

Note: Do not exceed the recommended sample input volume of 10 mL.

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25oC) for up to 2 years without showing any reduction in performance.

 

 

Kit Components
Component Cat. 51300 (10 preps)
Lysis Buffer D 3 x 125 mL
Slurry A2 14 mL
Binding Buffer B 20 mL
Binding Buffer C 30 mL
Wash Solution A 3 x 38 mL
Elution Buffer B 15 mL
Midi Filter Spin Columns 10
Midi Collection and Elution Tubes 20
Spin Columns Inserted into Collection Tubes 10
Elution Tubes (1.7 mL) 10
Product Insert 1
Title Loa loa and Onchocerca ochengi miRNAs detected in host circulation
Journal Molecular and Biochemical Parasitology2014.
Authors Lucienne Tritten, Maeghan O’Neill, Samuel Wanji, Abdel Njouendoui, Fanny Fombad, Jonas Kengne-Ouaffo, Charles Mackenzie, Timothy Geary.