Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format)

For rapid and simple isolation of circulating RNA including exosomal RNA from plasma/serum samples

  • Isolate all sizes of circulating and exosomal RNA, including microRNA
  • Versatile plasma and serum input volumes (0.25 mL to 5 mL)
  • Concentrate circulating and exosomal RNA into small elution volumes
  • Isolate inhibitor-free circulating RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
Items Cat. # Size
Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) 42800 50 Preps

Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 5 mL. This kit is able to isolate all sizes of circulating and exosmal RNA, including microRNA, without the use of phenol or chloroform. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating and exosomal RNA from large sample volumes. RNA can be isolated from either fresh or frozen samples using this kit. This kit is suitable for the isolation of RNA from serum or plasma prepared from blood collected only on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications including RT-PCR.

Preparation time for a single sample is less than 40 minutes. The purified plasma/serum free-circulating and exosmal RNA is eluted in an elution solution that is compatible with real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Free-circulating plasma and serum RNA can serve as both tumor- and fetal-specific markers for cancer detection and prenatal diagnosis. As well, free-circulating RNAs have the potential to provide biomarkers for other disease states. Free-circulating RNA in plasma or serum are usually present as short fragments of less than 1000nt, and free-circulating miRNA (21nt) can also be found in plasma and serum.

Kit Specifications
Minimum Plasma/Serum Input
0.25 mL
Maximum Plasma/Serum Input
5 mL
Size of RNA Purified
All sizes, including
microRNA
Time to Complete Purification
< 40 minutes

Storage Conditions and Product Stability 
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance. It is recommended to warm Slurry C2 and Lysis Buffer for 20 minutes at 60°C if any salt precipitation is observed.

 

 

Kit Components
Component Cat. 42800 (50 preps)
Slurry C2 14 mL
Lysis Buffer A 2 x 130 mL
Wash Solution A 38 mL
Elution Solution A 6 mL
Mini Filter Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1
Title Data on individual PCR efficiency values as quality control for circulating miRNAs
Journal Data in Brief. 2015.
Authors Anna Brunet-Vega, Carles Pericay, María Elisa Quílez, María José Ramírez-Lázaro, Xavier Calvet, Sergio Lario
Title Plasma MicroRNAs as Novel Biomarkers for Patients with Intraductal Papillary Mucinous Neoplasms of the Pancreas
Journal Cancer Prevention Research. 2015.
Authors Jennifer Permuth-Wey, Dung-Tsa Chen, William J. Fulp, Sean J. Yoder, Yonghong Zhang, Christina Georgeades, Kazim Husain, Barbara Ann Centeno, Anthony M. Magliocco, Domenico Coppola, and Mokenge Malafa
Title Blocking of targeted microRNAs from next-generation sequencing libraries
Journal Nucleic Acids Research. 2015.
Authors Brian S. Roberts, Andrew A. Hardigan, Marie K. Kirby, Meredith B. Fitz-Gerald, C. Mel Wilcox, Robert P. Kimberly and Richard M. Myers
Title Plasma circulating-microRNA profiles are useful for assessing prognosis in patients with cytogenetically normal myelodysplastic syndromes.
Journal Modern Pathology2014.
Authors Zhuang Zuo, Sourindra Maiti, Shimin Hu, Sanam Loghavi, George A Calin, Guillermo Garcia-Manero, Hagop M Kantarjian, L Jeffrey Medeiros, Laurence JN Cooper and Carlos E Bueso-Ramos.
Title Exosomes provide a protective and enriched source of miRNA for biomarker profiling compared to intracellular and cell-free blood.
Journal Journal of Extracellular Vesicles2014.
Authors Cheng L, Sharples RA, Scicluna BJ, Hill AF.
Title Three-Mode Electrochemical Sensing of Ultra-Low MicroRNA Levels.
Journal Journal of the American Chemical Society2013.
Authors Labib M, Khan N, Ghobadloo SM, Cheng J, Pezacki JP, Berezovski MV.