Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit

  • Efficient purification of DNA from large plasma and serum input volumes (5-10 mL)
  • Rapid and simple purification of all sizes of circulating DNA from plasma and serum samples
  • Two kits in one - purify and concentrate DNA from bodily fluids using a convenient two column system
  • Concentrate circulating DNA into a flexible elution volume ranging from 50 µL - 100 µL
  • Isolate inhibitor-free cell-free circulating DNA
  • Compatible with Streck Cell-Free DNA BCT® Tubes
Items Cat. # Size
Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit 55800 10 preps

Plasma/Serum Cell-Free Circulating DNA Purification Maxi

This kit provides a fast, reliable and convenient method to purify and concentrate using a two column system for the isolation of high quality, high purity and inhibitor-free cell-free circulating DNA (cfc-DNA) from fresh or frozen plasma/serum sample volumes ranging from 5 mL- 10 mL.

The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL. All components for the purification & concentration are provided in one convenient & fast kit for the easy processing of large input volumes of bodily fluids. For a schematic workflow of the protocol click here.

The purified plasma/serum cfc-DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, Southern Blot analysis, microarrays and NGS for various analyses including Fetal DNA screening, genotyping and other oncology and autoimmune disease research.

Background

Plasma/Serum cell-free circulating DNA (cfc-DNA) has the potential to provide biomarkers for certain cancers and disease states as well as fetal DNA in maternal blood. Currently, significant advancements are being made in utilizing cfc-DNA as biomarkers in the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases. Cell-free mitocondrial DNA (cfmtDNA) is also under investigation for its clinical significance. This cfc-DNA is usually present as short fragments of less than 1000 bp. In addition, cell-free fetal DNA has been widely used as a non-invasive method for prenatal diagnosis including early identification of fetal sex, genetic studies for families at high risk for inherited genetic disorders, screening for Rhesus factor, screening for aneuploidy and identification of preeclampsia.

Protocol

Kit Specifications
Minimum Plasma/Serum Input 5 mL
Maximum Plasma/Serum Input 10 mL
Size of DNA Purified All sizes
Elution Volume 50-100 μL
Time to Complete 10 Purifications 40-45 minutes
Average Yields* Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

 

 

Kit Components
Component Cat. 55800 (10 preps)
Binding Buffer B 2 x 85 mL
Proteinase K 1.2 mL
Wash Solution A 18 mL
Elution Buffer B 30 mL
Mini Spin Columns 10
Maxi Spin Columns 10
Collection Tubes 10
Elution Tubes (1.7 mL) 10
Product Insert 1