Plant/Fungi Total RNA Purification Kit

For the rapid purification of total RNA (including microRNA) from plants and fungi

  • Extract total RNA, including virus & viroid RNA
  • Robust lysis buffer is well-suited to even challenging samples such as pine needle, grape leaf, etc
  • Isolate total RNA (including microRNA) without phenol
  • Isolated RNA is of high quality, integrity and diversity
  • Also available in 96-well format for high throughput applications
Items Cat. # Size
Plant/Fungi Total RNA Purification Kit 25800 50 preps
Plant/Fungi Total RNA Purification 96-Well Kit 31900 2 x 96-well plates
Plant/Fungi Total RNA Purification Kit 31350 100 preps

Plant/Fungi Total RNA Purification Kit

Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient.

The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen's Plant/Fungi Total RNA Purification Kit is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.



Kit Specifications - Spin Column
Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Size of RNA Purified

All sizes, including small RNA (< 200 nt)

Maximum Amount of Starting Material:
Plant Tissues
Plant Cells
50 mg
1 x 106 cells
50 mg (wet weight)
Average Yields*
50 mg Tomato Leaves
50 mg Tabacco Leaves
50 mg Plum Leaves
50 mg Grape Leaves
50 mg Peach Leaves
60 μg
60 μg
32 μg
35 μg
30 μg
Time to Complete 10 Purifications
30 minutes

* Yield will vary depending on the type of sample processed.

Kit Specifications - 96-well
Binding Capacity Per Well
50 μg
Maximum Loading Volume Per Well
500 μL
Size of RNA Purified

All sizes, including small RNA (< 200 nt)

Maximum Amount of Starting Material:
Plant Tissues
40 mg
40 mg
Average Yields*
25 mg of Grape Leaves
40 mg Tomato Leaves
40 mg Tobacco Leaves
40 mg Peach Leaves
5-7 μg
20-30 μg
20-30 μg
15-20 μg
Time to Complete 96 Purifications
30 minutes

* Yield will vary depending on the type of sample processed.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits

Tobacco (Nicotiana tabacum)
Aspergillus niger
Tomato (Lycopersicon esculentum)
Mucor racemosus
Pepper (Capsicum annuum)
Cladosporium cladosporioides
Potato (Solanum tuberosum) Fusarium oxysporum
Arabidopsis thaliana1 Penicillium sp.
Peach (Prunus persica) Botrytis cinerea (Botryotinia fuckeliana)
Apple (Malus sp.)
Pichia sp.
Pear (Pyrus sp.)
Rhizopus oryzae
Grape vine (Vitis sp.)
Alternaria tenuissima
Plum (Prunus sp.)  
Palm (Arecaceae)  
Pine needle (Pinaceae)  
Black berry
Persimmon (Ebenaceae)
Potato tuber (Solanum)  
Plum fruit
Vanilla bean
Cotton (Gossypium)  



Kit Components
Component Cat. 25800 (50 preps) Cat. 31350 (100 preps)
Lysis Buffer C 60 mL 1 x 20 mL, 1 x 60 mL
Wash Solution A 38 mL 38 mL
Elution Solution A 6 mL 6 mL
Filter Columns 50 100
Spin Columns 50 100
Collection Tubes 100 200
Elution Tubes (1.7 mL) 50 100
Product Insert 1 1
Component Cat. 31900 (2 x 96-well)  
Lysis Solution 2 x 40 mL  
Wash Solution 2 x 40 mL  
Elution Solution 20 mL  
96-Well Filter Plate 2  
Adhesive Tape 4  
96-Well Collection Plate 2  
96-Well Elution Plate 2  
Product Insert 1  


Title Analysis of Microtubule-Associated-Proteins during IBA-Mediated Adventitious Root Induction Reveals KATANIN Dependent and Independent Alterations of Expression Patterns
Journal PLOS ONE. 2015.
Authors Mohamad Abu-Abied, Inna Mordehaev, Gujulla B Sunil Kumar, Ron Ophir, Geoffrey O. Wasteneys, Einat Sadot
Title Identification of conserved drought-adaptive genes using a cross-species meta-analysis approach
Journal BMC Plant Biology. 2015.
Authors Lidor Shaar-Moshe, Sariel Hübner and Zvi Peleg
Title An assemblage of divergent variants of a novel putative closterovirus from American persimmon
Journal Virus Genes. 2015.
Authors Takao Ito, Akihiko Sato, Koichi Suzaki
Title COBRA-LIKE 2, a member of the GPI-anchored COBRA-LIKE family, plays a role in cellulose deposition in Arabidopsis seed coat mucilage secretory cells.
Journal Plant Physiology Preview2015.
Authors Ben-Tov D, Abraham Y, Stav S, Thompson K, Loraine A, Elbaum R, De Souza A, Pauly M, Kieber JJ, Harpaz-Saad S
Title Priming with a double-stranded DNA virus alters Brassica rapa seed architecture and facilitates a defense response
Journal Gene2014.
Authors Melanie L. Kalischuk, Dan Johnson, Lawrence M. Kawchuk
Title Sequence diversity and gene expression analyses of expansin-related proteins in the white-rot basidiomycete, Phanerochaete carnosa.
Journal Fungal Genetics and Biology2014.
Authors Hitoshi Suzuki, Thu V. Vuong, Yunchen Gong, Kin Chan, Chi-Yip Ho, Emma R. Master, Akihiko Kondo.
Title Expression analysis of histone acetyltransferases in rice under drought stress.
Journal Biochemical and Biophysical Research Communications2013.
Authors Fang H, Liu X, Thorn G, Duan J, Tian L.
Title Weak-acid preservatives: pH and proton movements in the yeast Saccharomyces cerevisiae.
Journal International Journal of Food Microbiology2012.
Authors Stratford M, Nebe-von-Caron G, Steels H, Novodvorska M, Ueckert J, Archer DB.
Title Identification and genetic characterization of a gibberellin 2-oxidase gene that controls tree stature and reproductive growth in plum.
Journal Journal of Experimental Botany2011.
Authors El-Sharkawy I, El Kayal W, Prasath D, Fernˆndez H, Bouzayen M, Svircev AM, Jayasankar S.
Title Analysis of Fungal Gene Expression by Real Time Quantitative PCR
Journal Methods in Molecular Biology, Molecular and Cell Biology Methods for Fungi 103-114. Humana Press.2010.
Authors Ish-Shalom1 S., Lichter A.
Title Molecular characterization of seven genes encoding ethylene-responsive transcriptional factors during plum fruit development and ripening
Journal The Journal of Experimental Botany.60: 907 - 9222009.
Authors El-Sharkawy I, Sherif S, Mila I, Bouzayen M, Jayasankar S.
Title Detection and genetic characterisation of a novel mycovirus in Hymenoscyphus fraxineus, the causal agent of ash dieback.
Journal Infection, Genetics and Evolution. 2014.
Authors Corine N. Schoebel, Stefan Zoller, Daniel Rigling.
Title Development of a Pseudomonas syringae–Eutrema salsugineum pathosystem to investigate disease resistance in a stress tolerant extremophile model plant.
Journal Plant Pathology. 2014.
Authors MTS Yeo, P Carella, J Fletcher, MJ Champigny, EA Weretilnyk and Cameron
Title Profiling microRNAs in Eucalyptus grandis reveals no mutual relationship between alterations in miR156 and miR172 expression and adventitious root induction during development.
Journal BMC Genomics. 2014.
Authors Aviv Levy, David Szwerdszarf, Mohamad Abu-Abied, Inna Mordehaev, Yossi Yaniv, Joseph Riov, Tzahi Arazi and Einat Sadot
Title Enhanced biosynthesis of bioactive abietane diterpenes by overexpressing AtDXS or AtDXR genes in Salvia sclarea hairy roots.
Journal Plant Cell, Tissue and Organ Culture (PCTOC). 2014.
Authors Mariacarmela Vaccaro, Nicola Malafronte, Mariaevelina Alfieri, Nunziatina De Tommasi, Antonietta Leone
Title The role of carbon starvation in the induction of enzymes that degrade plant-derived carbohydrates in Aspergillus niger.
Journal Fungal Genetics Biology. 2014.
Authors van Munster JM, Daly P, Delmas S, Pullan ST, Blythe MJ, Malla S, Kokolski M, Noltorp EC, Wennberg K, Fetherston R, Beniston R, Yu X, Dupree P, Archer DB.
Title A Contig-Based Strategy for the Genome-Wide Discovery of MicroRNAs without Complete Genome Resources.
Journal PLoS One. 2014.
Authors Wen JZ, Liao JY, Zheng LL, Xu H, Yang JH, Guan DG, Zhang SM, Zhou H, Qu LH.
Title The transcription factor SlSHINE3 modulates defense responses in tomato plants.
Journal Plant Mol Biology. 2013.
Authors Buxdorf K, Rubinsky G, Barda O, Burdman S, Aharoni A, Levy M.
Title An Arabidopsis homolog of importin beta 1 is required for ABA response and drought tolerance.
Journal The Plant Journal. 2013.
Authors Luo Y, Wang Z, Ji H, Fang H, Wang S, Tian L, Li X.
Title Novel variants of grapevine leafroll-associated virus 4 and 7 detected from a grapevine showing leafroll symptoms.
Journal Archives of Virology. 2013.
Authors Ito T, Nakaune R, Nakano M, Suzaki K.
Title Trancriptional landscape of Aspergillus niger at breaking of conidial dormancy revealed by RNA-sequencing.
Journal BMC Genomics. 2013.
Authors Novodvorska M, Hayer K, Pullan ST, Wilson R, Blythe MJ, Stam H, Stratford M, Archer DB.
Title Graft-induced Changes in MicroRNA Expression Patterns in Citrus Leaf Petioles.
Journal The Open Plant Science Journal. 2013.
Authors R. Tzarfati, S.Ben-Dor, I. Sela and E.E. Goldschmidt
Title Silencing of the Host Factor eIF(iso)4E Gene Confers Plum Pox Virus Resistance in Plum.
Journal PLOS One. 2013.
Authors Wang X, Kohalmi SE, Svircev A, Wang A, Sanfaçon H, Tian L.
Title Gene expression indicates a zone of heterocyst differentiation within the thallus of the cyanolichen Pseudocyphellaria crocata.
Journal New Phytologist. 2012
Authors Chua JP, Wallace EJ, Yardley JA, Duncan EJ, Dearden PK, Summerfield TC.
Title Novel variants of grapevine leafroll-associated virus 4 and 7 detected from a grapevine showing leafroll symptoms.
Journal Archives of Virology. 2012
Authors Ito T, Nakaune R, Nakano M, Suzaki K.
Title A Survey of MicroRNA Length Variants Contributing to miRNome Complexity in Peach (Prunus Persica L.).
Journal Frontiers in Plant Science. 2011
Authors Colaiacovo M, Bernardo L, Centomani I, Crosatti C, Giusti L, Orrù L, Tacconi G, Lamontanara A, Cattivelli L, Faccioli P.
Title LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues.
Journal BMC Research Notes. 2011
Authors Yaffe H, Buxdorf K, Shapira I, Ein-Gedy S, Moyal-Ben Zvi M, Fridman E, Moselion M, Levy M.
Title Root colonisation of Pseudomonas aeruginosa strain UPMP3 and induction of defence-related genes in oil palm (Elaeis guineensis).
Journal Annals of Applied Biology. 2011
Authors Sathyapriya H, Sariah M, Siti Nor Akmar A and Wong M.
Title A classification of Pepper yellow mosaic virus isolates into pathotypes.
Journal European Journal of Plant Pathology. 2011
Authors Moura M, Mituti T, Marubayashi J, Gioria R, Kobori R, Pavan M, Silva N, Krause-Sakate R.
Title Regulation of two germin-like protein genes during plum fruit
Journal Journal of Experimental Botony, Mar 4 2010.
Authors El-Sharkawy I, Mila I, Bouzayen M and Jayasankar S.
Title Arabidopsis DNA methyltransferase AtDNMT2 associates with histone deacetylase AtHD2s activity.
Journal Biochemical and Biophysical Research Communications, p103-114. Mar 21 2010.
Authors Song Y, Wu K, Dhaubhadel S, An L, Tian L.