Norgen's Blood RNA Preservation and Purification System

A Complete System for the Preservation and Isolation of Whole Blood RNA

  • Input blood volumes of 3 mL
  • Preservation of whole blood RNA for up to 12 days at room temperature
  • Excellent RNA purity (A260/280 ratio >1.8)
  • Isolate 6 to 25 μg of RNA from 3 mL blood
  • No enzymatic treatment required
  • No specialized equipment necessary
  • Minimize induction of gene expression
  • Fast and convenient spin column protocol
Items Cat. # Size
Norgen's Blood RNA Preservation Tubes and RNA Purification System 52720 96 preps
Norgen's Blood RNA Preservation Tubes and RNA Purification System 52620 48 preps

Norgen's Blood RNA Preservation and Purification System

This kit provides a complete system for the collection, stabilization, storage, and transportation of whole blood specimens followed by a rapid and efficient protocol for purification of intracellular RNA. The system requires the use of Norgen’s Blood RNA Preservative Tubes for blood collection and RNA stabilization, followed by RNA purification using convenient spin columns.

blood tubes 

Sample Collection and Stabilization

Norgen’s Blood RNA Preservative Tubes (included in kit) feature an aqueous preservative that protects RNA molecules from degradation by RNases and minimizes induction of gene expression. Norgen’s Blood RNA Preservative Tubes are intended for the collection of whole blood and stabilization of cellular RNA for up to 12 days at 18–25°C or up to 21 days at 2–8°C. Studies evaluating stability for longer time periods at other temperatures are ongoing, and further information regarding these findings can be obtained by contacting Norgen Biotek. The actual duration of RNA stabilization may vary depending upon the species of cellular RNA and the downstream application used. Five different transcripts were validated for stabilization specifications (IL-16, NFKβIA, RAS1, FOS and IL1B gene transcripts).

RNA Concentration and Purification

Norgen’s Blood RNA Preservation and Purification Kit provides a rapid method for the isolation and purification of total RNA from 3 mL of human whole blood collected in Norgen’s Blood RNA Preservative Tubes. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Background

Collection of whole blood is the first step in many molecular assays used to study cellular RNA. However, a major problem in such experiments is the instability of the cellular RNA profile in vitro. Studies have shown that the copy numbers of individual mRNA species in whole blood can change more than 1000-fold during storage or transport at room temperature, due to rapid RNA degradation and induced expression of certain genes after the blood is drawn. Such changes in the RNA expression profile make reliable studies of gene expression impossible. A method that preserves the RNA expression profile is therefore essential for accurate analysis of gene expression in human whole blood.

Kit Specifications
Volume of Blood Collected
3 mL
Volume of Blood-Preservative Mix
9 mL
Preservation Time/Temperatures
12 days at 18-25°C
21 days at 4-8°C
Maximum Spin Column Binding Capacity
50 μg
Maximum Spin Column Loading Capacity
650 μL
Size of RNA Purified
All sizes, including
small RNA (< 200 nt)
Time to Complete 10 Purifications
45 minutes
Average Yield
6 - 25 μg per 3 mL preserved human blood

Storage Conditions and Product Stability

The Blood RNA Preservative, RNA Extraction Buffer A and RNA Extraction Buffer B should be kept tightly sealed and stored at 4oC upon arrival for up to 1 year without showing any reduction in performance. The rest of the buffers should be kept tightly sealed and stored at room temperature (15-25oC) for up to 1 year without showing any reduction in performance.

 
For Research Use Only. Not for use in diagnostics procedures.