microRNA Purification Kit

Rapid purification of microRNA without phenol; isolation and extraction of microRNA

  • Extract high quality & purity miRNA in 30 minutes
  • Two column kit- eliminate large RNA on first column and capture microRNA on a second column
  • Elute microRNA in 25 µL ready for miRNA profiling
  • Process a wide range of samples- cell, tissue, bacteria, bodily fluids, etc
Items Cat. # Size
microRNA Purification Kit 21300 25 preps

 

microRNA Purification Kit

This kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured animal cells, small tissue samples, bacterial cells, plants and blood.

Two columns are provided with this kit. The first column captures large RNA, while the small RNA are captured on a second column and are eluted concentrated in 25 µL of nuclease-free water.

The small RNA can be used in various downstream applications relating to miRNA profiling, gene regulation and functional analysis. The eluted RNA is ready for RT-qPCR, microarrays, and NGS applications.

Background

These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation.

 

microRNA Purification Kit

This kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured animal cells, small tissue samples, bacterial cells, plants and blood.

Two columns are provided with this kit. The first column captures large RNA, while the small RNA are captured on a second column and are eluted concentrated in 25 µL of nuclease-free water.

The small RNA can be used in various downstream applications relating to miRNA profiling, gene regulation and functional analysis. The eluted RNA is ready for RT-qPCR, microarrays, and NGS applications.

Background

These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation.

Title Human microRNAs profiling in response to influenza A viruses (subtypes pH1N1, H3N2, and H5N1)
Journal Experimental Biology and Medicine. 2015.
Authors Makkoch J, Poomipak W, Saengchoowong S, Khongnomnan K, Praianantathavorn K, Jinato T, Poovorawan Y, Payungporn S
Title Circulating microRNA Profiling Needs Further Refinement Before Clinical Use in Patients With Aortic Stenosis
Journal Valvular Heart Disease. 2015.
Authors Sean Coffey, MBBS, Michael J. A. Williams, MD, L. Vicky Phillips, Gregory T. Jones, PhD
Title Identification of MicroRNAs and Transcript Targets in Camelina sativa by Deep Sequencing and Computational Methods
Journal PLoS ONE2015.
Authors Saroj Poudel, Niranjan Aryal, Chaofu Lu
Title MicroRNA-181b regulates ALX/FPR2 expression and proresolution signaling in human macrophages
Journal The Journal of Biological Chemistry2014.
Authors Anna Maria Pierdomenico, Antonio Recchiuti, Felice Simiele, Marilina Codagnone, Veronica Cecilia Mari, Giovanni Davi and Mario Romano
Title Role of TLR13 in innate immune recognition of group B streptococci
Journal Infection and Immunity2014.
Authors Giacomo Signorino, Nastaran Mohammadi, Francesco Patanè, Marco Buscetta, Mario Venza, Isabella Venza, Giuseppe Mancuso, Angelina Midiri, Lena Alexopoulou, Giuseppe Teti, Carmelo Biondo and Concetta Beninati.
Title Quantification of Circulating miRNAs in Plasma: Effect of Preanalytical and Analytical Parameters on Their Isolation and Stability.
Journal The Journal of Molecular Diagnostics2013.
Authors Sourvinou IS, Markou A, Lianidou ES.
Title A Panel of four MicroRNAs accurately differentiates Malignant from Benign Indeterminate Thyroid Lesions on Fine Needle Aspiration.
Journal Clinical Cancer Research2012.
Authors Xavier M. Keutgen, Filippo Filicori, Michael J. Crowley, et al.
Title Effective inhibition of replication of infectious bursal disease virus by miRNAs delivered by vectors and targeting the VP2 gene
Journal Journal of Virological Methods.65(2):127-322010.
Authors Wang Y, Sun H, Shen P, Zhang X, Xia X, Xia B.
Title Bacterial recognition by TLR7 in the lysosomes of conventional dendritic cells
Journal Nature Immunology.10,587 - 5942009.
Authors Mancuso G,Gambuzza M,Midiri A,Biondo C,Papasergi S,Akira S,Teti G, Beninati C.
Title Novel expression of microRNAs in serum samples of Iraqi breast cancer women
Journal American Journal of BioMedicine. 2014.
Authors Zaynab Saad; Muhammed Arif; Nahi Yassen; Hameed Jasim; Majed Jelawe; James Brown
Title Expanding the annotation of zebrafish microRNAs based on small RNA sequencing
Journal Gene. 2014.
Authors Thomas Desvignes, Michael J. Beam, Peter Batzel, Jason Sydes, John H. Postlethwait
Title AcvR1-mediated BMP signaling in second heart field is required for arterial pole development: Implications for myocardial differentiation and regional identity.
Journal Developmental Biology. 2014.
Authors Thomas PS, Rajderkar S, Lane J, Mishina Y, Kaartinen V.
Title Effective inhibition of infectious bursal disease virus replication by 1recombinant avian adeno-associated virus-delivered microRNAs.
Journal Journal of Genetic Virology. 90: 1417-1422.
Authors Wang Y, Sun H, Shen P, Zhang X, Xiaoli X.