Microm DNA Isolation Kit

  • Isolate high quality total DNA from a variety of air filter types
  • Fast and easy processing using rapid spin-column format
  • Small elution volume of 20 µL
  • Isolate total DNA without compromising total yield
  • No phenol or chloroform extractions
Items Cat. # Size
Microm DNA Isolation Kit 55300 50 preps
Microm DNA Isolation Kit 55350 100 preps
Microm DNA Isolation 96-Well Kit 55900 2 x 96-well plates

Microm DNA Isolation Kit

Norgen’s Microm DNA Isolation Kit is designed for the rapid preparation of genomic DNA from filters used for air sample collection. Various air-borne microorganisms may be trapped on the filter, including bacteria, fungi and viruses. Genomic DNA is efficiently extracted from the filter by a combination of heat treatment and a unique lysis buffer. PCR inhibitors are effectively removed during the washing steps and the purified genomic DNA is ready to use for downstream applications such as PCR and microarray analysis. The option of bead beating is also provided in order to allow for improved DNA yields for certain fungal and yeast species captured on the filter. Preparation time for ten samples is less then 30 minutes, and each kit contains sufficient materials for 50 or 100 preparations.

Kit Specifications - Spin Column
Sample Type
Dried or preserved filter
Maximum Column Binding Capacity
10 μL
Maximum Column Loading Volume
650 μg
Minimum Elution Volume 20 μL
Size of DNA Purified All sizes
Time to Complete Purification
20 minutes
Kit Specifications - 96-well
Sample Type
Dried or preserved filter
Maximum Binding Capacity
50 μL
Maximum Loading Volume
750 μg
Size of DNA Purified All sizes
Time to Complete 96 Purifications
50 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.

 

 

Kit Components
Component Cat. 55300 (50 preps) Cat. 55350 (100 preps)
Lysis Buffer B 40 mL 2 x 40 mL
Wash Solution A 38 mL 2 x 38 mL
Elution Buffer B 15 mL 2 x 15 mL
Micro Spin Columns 50 100
Collection Tubes 50 100
Elution Tubes (1.7 mL) 50 100
Product Insert 1 1
     
Component Cat. 55900 (2 x 96-well)  
Lysis Buffer B 2 x 110 mL  
Wash Solution A 2 x 38 mL  
Elution Buffer B 2 x 15 mL  
96-Well Plate 2  
96-Well Collection Plate 2  
Adhesive Tape 4  
96-Well Elution Plate 2  
Product Insert 1