In Vitro Transcription Clean-Up and Concentration Mini Kit

  • Clean-up and concentrate transcripts of all sizes - both large and small
  • Complete column purification - quick and convenient
  • Cleans in vitro transcription reaction mixtures removing buffers, unincorporated NTPs and enzymes
  • Rapid procedure is complete in 15 minutes
  • Provides high quality RNA transcripts for any application
Items Cat. # Size
In Vitro Transcription Clean-Up and Concentration Mini Kit 53000 25 preps

 


In Vitro Transcription Clean-Up and Concentration Mini Kit

This kit provides a rapid, simple and efficient procedure for the purification and concentration of up to 50 µg of RNA transcripts from in vitro transcription reactions. The kit purifies RNA transcripts from the various impurities used during the in vitro transcription reaction, including polymerases, unincorporated dNTPs, salts and DTT, without the use of phenol, chloroform or alcohol precipitation. The kit is robust with transcripts > 100b and provides a high quality product with up to 90% recovery. The purified transcripts can be used in a number of downstream applications including RT-PCR, qRT-PCR and more.

 

 

Kit Specifications
Column Binding Capacity
50 μg
Maximum Column Loading Volume
600 μL
Size of RNA Purified
> 100b
Time to Complete 10 Purifications
15 minutes
Maximum Amount of Starting Material
50 μg RNA
Minimum Elution Volume
30 μL

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance.