In Vitro Transcription Clean-Up and Concentration Micro Kit

  • Clean-up and concentrate transcripts of all sizes - both large and small
  • Complete column purification - quick and convenient
  • Cleans in vitro transcription reaction mixtures removing buffers, unincorporated NTPs and enzymes
  • Rapid procedure is complete in 15 minutes
  • Provides high quality RNA transcripts for any application
Items Cat. # Size
In Vitro Transcription Clean-Up and Concentration Micro Kit 52900 25 preps


In Vitro Transcription Clean-Up and Concentration Micro Kit

This kit provides a rapid, simple and efficient spin column procedure for the purification and concentration of up to 35 µg of RNA transcripts from in vitrotranscription reactions. The kit purifies RNA transcripts from the various impurities used during the in vitro transcription reaction, including polymerases, unincorporated dNTPs, salts and DTT, without the use of phenol, chloroform or alcohol precipitation. The kit is robust with transcripts > 100b and provides a high quality product with up to 90% recovery. The purified transcripts are of the highest quality and can be used in a number of downstream applications including qRT-PCR, RT-PCR and more. This procedure can be completed in approximately 15 minutes.

 

 

Kit Specifications
Column Binding Capacity
35 μg
Maximum Column Loading Volume
600 μL
Size of RNA Purified
> 100b
Time to Complete 10 Purifications
15 minutes
Maximum Amount of Starting Material
35 μg RNA
Minimum Elution Volume
15 μL

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance.

 

 

Product Information Sheets: 
 In Vitro Transcription Clean-Up and Concentration Micro Kit - Product Information Sheet