Genomic DNA Isolation Kit

For the isolation of genomic DNA from animal tissues, cells, bodily fluids, virus and swabs

  • Isolate genomic DNA from animal tissues, cells, bodily fluids, viruses and swabs
  • Rapid and convenient spin column procedure
  • Purified DNA is of the highest quality and integrity for sensitive downstream applications including PCR, qPCR, genotyping, sequencing and more
Items Cat. # Size
Genomic DNA Isolation Kit 24700 50 preps
Genomic DNA Isolation Kit 24750 100 Preps

Genomic DNA Isolation Kit

This kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, viruses, bodily fluids and swabs using a rapid spin column protocol. Purified DNA is of an excellent yield and quality, and is immediately ready for any downstream application including PCR, qPCR, genotyping, sequencing and more. The protocol can be completed in approximately 80 minutes (including incubation time).

Genomic DNA Isolation Kit Figure 2

Kit Specifications
Column Binding Capacity
25 µg
Average Yield:*
HeLa Cells (1 x 106)
Tissue (from 10 mg kidney)
8 µg
10 µg
Maximum Amount of Starting Material:
Animal Tissues
Cultured Cells
Bodily Fluids (blood, saliva)
Viral Suspension
20 mg
3 x 106 cells
150 µL
150 µL
Time to Complete 10 Purifications
80 minutes

* Yield will vary depending on the type of sample processed

Storage Conditions and Product Stability
The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.

 

 

Kit Components
Component Cat. 24700 (50 preps) Cat. 24750 (100 preps)
Digestion Buffer A 25 mL 2 x 25 mL
Buffer SK 30 mL 2 x 30 mL
Wash Solution A 18 mL 2 x 18 mL
Elution Buffer B 30 mL 2 x 30 mL
Proteinase K 12 mg 2 x 12 mg
Spin Columns 50 100
Collection Tubes 50 100
Elution Tubes (1.7 mL) 50 100
Product Insert 1 1
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Title Multiplex PCR assay for the simultaneous detection of C. perfringensP. aeruginosa and K. pneumoniae
Journal The Official Journal of The Royal College of Pathogens. 2015.
Authors Pradeepkiran Jangampalli Adi, Ramesh Babu Pappithi, Praveen Chakravarthi Veeraraghavulu, Bhaskar Matcha
Title Spatial and temporal dynamics of virus occurrence in two freshwater lakes captured through metagenomic analysis
Journal Frontiers in Microbiology. 2015.
Authors Mohammad Mohiuddin and Herb E. Schellhorn
Title Epigenetic modulations in early endothelial cells and DNA hypermethylation in human skin after sulfur mustard exposure
Journal Toxicology Letters. 2015.
Authors Steinritz D, Schmidt A, Balszuweit F, Thiermann H, Simons T, Striepling E, Bölck B, Bloch W
Title Epigenome data release: a participant-centered approach to privacy protection
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Authors Stephanie O. M. Dyke, Warren A. Cheung,# Yann Joly, Ole Ammerpohl, Pavlo Lutsik, Mark A. Rothstein, Maxime Caron, Stephan Busche, Guillaume Bourque, Lars Rönnblom, Paul Flicek, Stephan Beck, Martin Hirst, Henk Stunnenberg, Reiner Siebert, Jörn Walter, and Tomi Pastinen
Title Two Gonostomatid Ciliates from the Soil of Lombardia, Italy; including Note on the Soil Mapping Project
Journal Journal of Eukaryotic Microbiology. 2015.
Authors Daizy Bharti, Santosh Kumar and Antonietta La Terza

Title

Specific molecular signatures predict decitabine response in chronic myelomonocytic leukemia
Journal The Journal of Clinical Investigation2015.
Authors Kristen Meldi, Tingting Qin, Francesca Buchi, Nathalie Droin, Jason Sotzen, Jean-Baptiste Micol, Dorothée Selimoglu-Buet, Erico Masala, Bernardino Allione, Daniela Gioia, Antonella Poloni, Monia Lunghi, Eric Solary, Omar Abdel-Wahab, Valeria Santini and Maria E. Figueroa

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Impacts of Degraded DNA on Restriction Enzyme Associated DNA Sequencing (RADSeq)
Journal Molecular Ecology Resources2015.
Authors Graham CF, Glenn TC, McArthur AG, Boreham DR, Kieran T, Lance S, Manzon RG, Martino JA, Pierson T, Rogers SM, Wilson JY, Somers CM.

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Association of azoospermia factor region deletions in infertile male subjects among Malaysians.
Journal Andrologia2014.
Authors Hussein AA, Vasudevan R, Patimah I, Prashant N, Nora FA.

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Lack of association between UBE2E2 gene polymorphism (rs7612463) and type 2 diabetes mellitus in a Saudi population.
Journal ACTA Biochimica Polonica. 2014.
Authors Alharbi KK, Khan IA, Al-Sheikh YA, Alharbi FK, Alharbi FK, Al-Nbaheen MS.
Title RNF8-Independent Lys63 Poly-Ubiquitylation Prevents Genomic Instability in Response to Replication-Associated DNA Damage
Journal PLOS ONE2014.
Authors Ramaekers CH, van den Beucken T, Bristow RG, Chiu RK, Durocher D, Wouters BG.
Title Genetic Structure and Natal Origins of Immature Hawksbill Turtles (Eretmochelys imbricata) in Brazilian Waters
Journal PLOS ONE2014.
Authors Proietti M, Reisser J, Marins L, Rodriguez-Zarate C, Marcovaldi M, Monteiro D, Pattiaratchi C, Secchi E.
Title TLR4 Gene Polymorphism in Patients with Nonalcoholic Fatty Liver Disease in Comparison to Healthy Controls
Journal Metabolic Syndrome and Related Disordorders2014.
Authors Kiziltas S, Ata P, Colak Y, Mesçi B, Senates E, Enc F, Ulasoglu C, Tuncer I, Oguz A.
Title Hawksbill × loggerhead sea turtle hybrids at Bahia, Brazil: where do their offspring go?
Journal PeerJ2014.
Authors Maira C. Proietti, Julia Reisser, Luis F. Marins4, Maria A. Marcovaldi, Luciano S. Soares, Danielle S. Monteiro1, Sarath Wijeratne, Charitha Pattiaratchi, Eduardo R. Secchi.
Title Retrotransposon Hypomethylation in Melanoma and Expression of a Placenta-Specific Gene
Journal PLOS ONE2014.
Authors Macaulay EC, Roberts HE, Cheng X, Jeffs AR, Baguley BC, Morison IM
Title A54T polymorphism in the fatty acid binding protein 2 studies in a Saudi population with type 2 diabetes mellitus
Journal Lipids in Health and Disease2014.
Authors Alharbi KK, Khan IA, Bazzi MD, Al-Daghri NM, Hasan TN, Alnbaheen MS, Alharbi FK, Al-Sheikh YA, Syed R, Aboul-Soud MA.
Title Genetic, physiological and biochemical characterization of Bacillus sp. strain RMB7 exhibiting plant growth promoting and broad spectrum antifungal activities
Journal Microbial Cell Factories2014.
Authors Saira Ali, Sohail Hameed, Asma Imran, Mazhar Iqbal and George Lazarovits.
Title Prevalence of glucose-6-phosphate dehydrogenase deficiency and the role of the A- variant in a Saudi population
Journal Journal of International Medical Research2014.
Authors Alharbi KK, Khan IA
Title Shorter telomeres in peripheral blood mononuclear cells from older persons with sarcopenia: results from an exploratory study
Journal Frontiers in Aging Neuroscience2014.
Authors Emanuele Marzetti, Maria Lorenzi, Manuela Antocicco, Stefano Bonassi, Michela Celi, Simona Mastropaolo, Silvana Settanni, Vanessa Valdiglesias, Francesco Landi, Roberto Bernabei and Graziano Onder
Title Association of angiotensin converting enzyme gene insertion/deletion polymorphism and familial hypercholesterolemia in the Saudi population.
Journal Lipids Health and Disease2013.
Authors Alharbi KK, Kashour TS, Al-Hussaini W, Al-Nbaheen MS, Mohamed S, Hasanato RM, Tamimi W, Al-Naami MY, Khan IA.
Title The presence of methylation quantitative trait Loci indicates a direct genetic influence on the level of DNA methylation in adipose tissue
Journal PLOS ONE2013.
Authors Drong AW, Nicholson G, Hedman AK, Meduri E, Grundberg E, Small KS, Shin SY, Bell JT, Karpe F, Soranzo N, Spector TD, McCarthy MI, Deloukas P, Rantalainen M, Lindgren CM; MolPAGE Consortia.
Title ABCA1 C69T gene polymorphism and risk of type 2 diabetes mellitus in a Saudi population
Journal Journal of Bioscience2013.
Authors Alharbi K, Khan I, Al-Daghri N, Munshi A, Sharma V, Mohammed A, Wani K, Al-Sheikh Y, Al-Nbaheen M, Ahmed Ansari M, Syed R.
Title Global Analysis of DNA Methylation Variation in Adipose Tissue from Twins Reveals Links to Disease-Associated Variants in Distal Regulatory Elements
Journal The American Journal of Human Genetics2013.
Authors Grundberg E, Meduri E, Sandling J, Hedman A, Keildson S, Buil A, Busche S, Yuan W, Nisbet J, Sekowska M, Wilk A, Barrett A, Small K, Ge B, Caron M, Shin S, the Multiple Tissue Human Expression Resource Consortium, Lathrop M, Dermitzakis E, McCarthy M, Spector T, Bell J and Deloukas P.
Title Codon 54 in the Fatty Acid–binding Protein-2 (A54T) Polymorphism Studies in Type 2 Diabetes Mellitus in Saudi Population
Journal Diabetes & Metabolism2013.
Authors Alharbi K, Bazzi M, Al-Daghri N, Khan I, Al-Nbaheen M, Syed R, Atieh M and Ansari M
Title Molecular predictors of response to decitabine in advanced chronic myelomonocytic leukemia: a phase 2 trial.
Journal Blood2011.
Authors Braun T, Itzykson R, Renneville A, de Renzis B, Dreyfus F, Laribi K, Bouabdallah K, Vey N, Toma A, Recher C, Royer B, Joly B, Vekhoff A, Lafon I, Sanhes L, Meurice G, Oréar C, Preudhomme C, Gardin C, Ades L, Fontenay M, Fenaux P, Droin N, Solary E; Groupe Francophone des Myélodysplasies.
Title Aberrant DNA Methylation of Matrix Remodeling and Cell Adhesion Related Genes in Pterygium
Journal PLOS ONE2011.
Authors Riau AK, Wong TT, Finger SN, Chaurasia SS, Hou AH, Chen S., Yu SJ,Tong L.
Title Developing a rapid, efficient and low cost method for rapid DNA extraction from arthropods.
Journal SciFlow2011.
Authors Nicolˆs Oliveira Mega and LuÍs Fernando Revers.
Title Gene Expression and Genetic Variation Data Implicate PCLO in Bipolar Disorder.
Journal Biological Psychiatry2010.
Authors Choi KH, Higgs BW, Wendland JR, Song J, McMahon FJ, Webster MJ.