This kit provides a rapid method for the isolation and purification of total RNA (including microRNA) and DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Alternatively, the kit can be used to isolate total RNA alone or genomic DNA alone from FFPE tissue samples through varying the protease digestion time and performing optional RNase or DNAse digestions. Nucleic acids recovered with this kit are suitable for further downstream analysis by PCR, qPCR, microarrays, sequencing etc.
Norgen Biotek also offers a kit to separate RNA and DNA from the same FFPE tissue sample. Please visit the product page for more details: FFPE RNA/DNA Purification Plus Kit #54300
Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE RNA/DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids.