Fatty Tissue RNA Purification Kit

For purification of total RNA (including microRNA) from animal tissues with high lipid content

  • Isolate high quality total RNA from hard to extract fatty tissues
  • Isolate total RNA, including microRNA (miRNA)
  • No phenol or chloroform extractions
  • Fast and easy processing using rapid spin-column format
  • Genomic DNA removal without the use of nucleases
Items Cat. # Size
Fatty Tissue RNA Purification Kit 36200 25 preps


Fatty Tissue RNA Purification Kit

This kit was designed to extract high quality and yield RNA from fatty tissues like brain, breast, adipose tissues, etc. Often tissues that have high fat contents like brain, breast tissues, etc. can lead to low quality and yield RNA with non-optimized kits.

This kit provides a rapid and optimized method for the isolation and purification of total RNA from animal tissues with high lipid content, including brain and adipose tissues. The kit purifies all sizes of RNA down to microRNA (miRNA) without the use of inhibitory phenol or chloroform. While the kit is optimized for fatty tissues, it can also be used for most mammalian tissue samples.

The purified RNA is of the highest integrity, and can be used in a number of downstream applications including RT-qPCR, microarrays, and NGS.

 

 

 

 

Kit Specifications
Maximum Column Binding Capacity
50 µg
Maximum Column Loading Volume
650 µL
Size of RNA Purified
All sizes, including small RNA
(<200 nt)
Maximum Amount of Starting Material:
Brain and Adipose Tissues
20 mg
Time to Complete 10 Purifications
50 minutes
Average Yields*
Brain (15 mg)
15 µg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

 

Title MicroRNA expression is altered in lateral septum across reproductive stages
Journal Neuroscience. 2015.
Authors Saul MC, Zhao C, Driessen TM, Eisinger BE, Gammie SC
Title Hypothalamic neuropeptide Y (NPY) gene expression is not affected by central serotonin in the rainbow trout (Oncorhynchus mykiss).
Journal Comparative Biochemistry and Physiology2013.
Authors Mancebo MJ, Ceballos FC, Perez-Maceira J, Aldegunde M