Endotoxin Removal Kit (Mini) - For DNA

For the rapid removal of endotoxins from previously purified DNA

  • Reduce endotoxin levels to 0.1 EU/µg DNA or less
  • Effectively remove endotoxins in as little as 20 minutes
  • Remove endotoxins from up to 25 µg of DNA
  • Rapid spin-column format
Items Cat. # Size
Endotoxin Removal Kit (Mini) - For DNA 22700 25 preps

Endotoxin Removal Kit (Mini) - For DNA

This kit is designed for the rapid spin column removal of endotoxins from up to 25 mg of previously purified DNA. Norgen's spin columns bind DNA while endotoxins, salts and other contaminants are washed away. This kit reduces endotoxins to 0.1 EU/mg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. The convenient spin column procedure can be completed in approximately 20 minutes and typical recovery of DNA is >90% of the starting sample.

About Endotoxins

Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.

Kit Specifications
Maximum DNA Input
25 μg
Final Endotoxin Level
≤ 0.1 EU/µg DNA
Size of DNA Purified
Up to 13,000 bp
Maximum DNA Volume Input
100 μL
Average Recovery
> 90%
Time to Complete 10 Purifications
20 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. All the reagents should remain stable for at least 1 year in their unopened containers.

 

 

Kit Components
Component Cat. 22700 (25 preps)
Buffer SK 15 mL
Wash Solution H 18 mL
Elution Buffer I 6 mL
Endotoxin Removal Solution 1.5 mL
Spin Columns 25
Collection Tubes 25
Elution Tubes (1.7 mL) 25
Product Insert 1
Title Transplantation of prokaryotic two-component signaling pathways into mammalian cells.
Journal Proceedings of the National Academy of Sciences2014.
Authors Jonathan Hansen, Erik Mailand, Krishna Kumar Swaminathan, Joerg Schreiber, Bartolomeo Angelici, and Yaakov Benenson.
Title Wheat amylase trypsin inhibitors drive intestinal inflammation via activation of toll-like receptor 4.
Journal The Journal of Experimental Medicine2012.
Authors Junker Y, Zeissig S, Kim SJ, Barisani D, Wieser H, Leffler DA, Zevallos V, Libermann TA, Dillon S, Freitag TL, Kelly CP, Schuppan D.
Title The costimulatory immunogen LPS induces the B-Cell clones that infiltrate transplanted human kidneys.
Journal Proceedings of the National Academy of Sciences of the United States of America. 2012.
Authors Grover RK, Cheng J, Peng Y, Jones TM, Ruiz DI, Ulevitch RJ, Glass JI, Dennis EA, Salomon DR, Lerner RA.