Endotoxin Removal Kit (Midi) - For DNA

For the rapid removal of endotoxins from up to 0.2 mg of previously purified DNA

  • Reduce endotoxin levels to 0.1 EU/µg DNA or less
  • Effectively remove endotoxins in as little as 30 minutes
  • Remove endotoxins from up to 200 µg of DNA
  • Rapid spin-column format
Items Cat. # Size
Endotoxin Removal Kit (Midi) - For DNA 52200 10 preps

Endotoxin Removal Kit (Midi) - For DNA

This kit is designed for the rapid spin column removal of endotoxins from up to200 mof previously purified DNA. Norgen's spin columns bind DNA while endotoxins, salts and other contaminants are washed away. This kit reduces endotoxins to 0.1 EU/mg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. The convenient spin column procedure can be completed in approximately 30 minutes and typical recovery of DNA is >90% of the starting sample.

About Endotoxins

Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.

Kit Specifications
Maximum DNA Input
0.2 mg
Final Endotoxin Level
≤ 0.1 EU/μg DNA
Size of DNA Purified
Up to 13,000 bp
Maximum DNA Volume Input
0.75 mL
Average Recovery
> 90%
Time to Complete 10 Purifications
30 minutes

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. All the reagents should remain stable for at least 1 year in their unopened containers.

 

 

Kit Components
Component Cat. 52200 (10 preps)
Buffer SK 60 mL
Wash Solution H 18 mL
Wash Solution F 12 mL
Elution Buffer I 12 mL
Endotoxin Removal Solution 1.5 mL
Precipitation Solution 1.5 mL
Spin Columns (assembled with collection tubes) 10
Elution Tubes (1.7 mL) 10
Product Insert 1