Dried Blood Spot (DBS) DNA Isolation Kit

Rapid preparation of total DNA from dried blood spots

  • High quality DNA, free from RNA contamination
  • Isolate genomic DNA from anticoagulated and untreated blood
  • Rapid and convenient spin column procedure
Items Cat. # Size
Dried Blood Spot (DBS) DNA Isolation Kit 36000 50 preps

Dried Blood Spot (DBS) DNA Isolation Kit

This kit is designed for the rapid preparation of total DNA from dried blood spots with a quick and convenient spin column. The Dried Blood Spot DNA Isolation Kit allows for the isolation of DNA from the blood of various species, including humans. Preparation time for a single sample is 30 minutes. The purified DNA is of high quality and is completely compatible with downstream applications including PCR, qPCR and more.

Figure 1.  Good Yield and Integrity of DNA

Kit Specifications
Maximum Blood Input
3 x 3 mm diameter punches
Average yield* (ng)
150 ng
Column Binding Capacity
> 50 µg
Time to Complete 10 Purifications
35 minutes

Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability 
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. The kit contains a ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

 

 

Kit Components
Component Cat. 36000 (50 preps)
Digestion Buffer B 6 mL
Lysis Buffer B 20 mL
Wash Solution WN 18 mL
Wash Solution A 18 mL
Elution Buffer B 15 mL
Proteinase K 1.2 mL
Micro Spin Columns 50
Collection Tubes 50
Elution Tubes 50
Product Insert 1