Direct DNA Extraction Kit (Bacteria)

  • Rapid procedure
  • Easy and convenient protocol
  • Reproducible
  • Applicable for high throughput detection platforms
Items Cat. # Size
Direct DNA Extraction Kit (Bacteria) 61500 50 preps

Direct DNA Extraction Kit (Bacteria)

Norgen's Direct DNA Extraction Kit (Bacteria) contains a Direct Pathogen Extraction Buffer (Buffer DL) and pre-filled bead tubes for the extraction of DNA from enriched Gram negative and positive bacteria from food and culture samples. The kit first allows for the extraction of DNA directly from bacteria using mechanical homogenization with Buffer DL. The cell debris is then removed by a short centrifugation and the clean supernatant is ready for use in PCR reactions. Buffer DL is designed to stabilize extracted DNA and inactivate PCR inhibitors to provide a rapid and economic solution for high throughput pathogen detection, particularly for Gram-positive bacteria such as Listeria spp. Norgen's Direct DNA Extraction Kit (Bacteria) is compatible with sensitive detection applications including end-point PCR and real-time PCR.

Direct DNA Extraction Kit (Bacteria)
Procedure

Direct DNA Extraction Step 1
1. Take 1 mL from enriched culture
 
Direct DNA Extraction Step 2
2. Pellet the cells and resuspend with 400 µL of Buffer DL
 
Direct DNA Extraction Step 3
3. Homogenize in a bead beater
 
Direct DNA Extraction Step 4
4. Remove the cell debris by centrifugation
 
Direct DNA Extraction Step 5
5. The clean supernatant can be used directly in PCR

Storage Conditions 
All kit components should be stored at room temperature (15–25°C).

 

 

Kit Components
Component Cat. 61500 (50 preps)
Direct Pathogen Extraction Buffer (Buffer DL) 2 x 25 mL
Bead Tubes 50
Product Insert 1
Title Microbial population dynamics and profiling of quorum sensing agents in membrane bioreactor
Journal International Biodeterioration & Biodegradation. 2015.
Authors Hira Waheed, Imran Hashmi, Sher Jamal Khan, Sang Ryoung Kim, Muhammad Arshad, Habib Nasir