Blood DNA Isolation Mini Kit

For the rapid preparation of high quality DNA from whole blood

  • Input volumes up to 200 µL
  • High yield and high quality DNA ready for any application
  • Fast and convenient spin column protocol
Items Cat. # Size
Blood DNA Isolation Mini Kit 46300 50 Preps

Blood DNA Isolation Mini Kit

Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. DNA can be isolated from the blood of various species, including humans. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations. The kit isolates genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA from blood samples. The purified DNA is of excellent quality and yield and completely compatible with downstream applications including PCR, qPCR, Sequencing, Genotyping, Virus Detection, Southern Blot analysis

Figure 1.  High Yields of DNA

Kit Specifications
Maximum Blood Input
200 µL
Column Binding Capacity
> 50 µg
Average Yield (200 µL of blood)
4-12 µg*
Time to Complete 10 Purifications
30 minutes

*Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. The kit contains a ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

 

 

Kit Components
Component Cat. 46300 (50 preps)
Lysis Buffer B 20 mL
Solution WN 18 mL
Wash Solution A 18 mL
Elution Buffer B 30 mL
Proteinase K 1.2 mL
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1
Title Screening for genetic mutations in LDLR gene with familial hypercholesterolemia patients in the Saudi population
Journal Acta Biochimica Polonica. 2015.
Authors Alharbi KK, Kashour TS, Al-Hussaini W, Nbaheen MS, Hasanato RM, Mohamed S, Tamimi W, Khan IA
Title Prevalence of glucose-6-phosphate dehydrogenase deficiency and the role of the A− variant in a Saudi population.
Journal Journal of International Medical Research. 2014.
Authors Khalid Khalaf Alharbi and Imran Ali Khan
Title Polymorphic variants of MnSOD Val16Ala, CAT-262 C < T and GPx1 Pro198Leu genotypes and the risk of laryngeal cancer in a smoking population.
Journal The Journal Laryngology and Otology. 2013.
Authors Aynali G, Doğan M, Sütcü R, Yüksel O, Yariktaş M, Unal F, Yasan H, Ceyhan B, Tüz M.
Title Genetic differentiation and inheritance of random amplified polymorphic DNA (RAPD) markers in pectoral spine phenotypic sub-groups of Clarias gariepinus.
Journal African Journal of Biotechnology. 2013.
Authors Oyebola O, Omitoyin B, Salako A, Ajani E, Awodiran M
Title Thrombophilic gene polymorphism studies in G6PD deficient individuals from Saudi population.
Journal Bioinformation. 2012.
Authors Alharbi KK, Khan IA, Syed R.
Title Investigation of PON 55 Gene Polymorphism Frequency in Patients with Hyperlipidemia.
Journal Atherosclerosis. 2012.
Authors Sanghera D, Saha N, Kamboh M