Blood DNA Isolation Midi Kit

For the rapid preparation of high quality DNA from 0.3 to 2 mL of whole blood

  • Input volumes from 0.3 to 2 mL
  • DNA is of excellent yield and quality
  • Fast and convenient spin column protocol
Items Cat. # Size
Blood DNA Isolation Midi Kit 51400 20 Preps

Blood DNA Isolation Midi Kit

Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. This kit allows for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.

Kit Specifications
Maximum Blood Input
2 mL
Column Binding Capacity
> 100 µg
Average Yield
60 µg*
Elution Volume
200-300 uL
Analyte Purified
Genomic DNA,
mitochondrial DNA,
viral DNA, bacterial DNA
Format
Spin Column
Time to Complete 10 Purifications
45 minutes

*Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. The kit contains a ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

 

 

Kit Components
Component Cat. 51400 (20 preps)
Lysis Buffer B 2 x 40 mL
Solution WN 55 mL
Wash Solution A 2 x 38 mL
Elution Buffer B 30 mL
Midi Spin Columns 20
Midi Collection Tubes 20
Midi Elution Tubes 20
Product Insert 1