Bacterial Genomic DNA Isolation Kit

For the rapid preparation of genomic DNA from bacteria

  • Isolate genomic DNA from all types of bacteria (both Gram-positive and Gram-negative)
  • Rapid and convenient spin column protocol
  • 96-well format available for high throughput
  • High yield, high quality DNA for sensitive downstream applications including sequencing, PCR, qPCR and more

Bacterial Genomic DNA Isolation Kit Figure 1

Items Cat. # Size
Bacterial Genomic DNA Isolation Kit 17900 50 preps
Bacterial Genomic DNA Isolation 96-Well Kit 17950 2 x 96-well plates

Bacterial Genomic DNA Isolation Kit

This kit is designed for the rapid spin column preparation of genomic DNA from 2 x 109 viable bacterial cells (between 0.5 and 1.0 mL of culture). This kit can be used for both Gram-negative and Gram-positive bacteria including Escherichia coli and Bacillus cereusPurified genomic DNA is of an excellent quality and yield, and is fully compatible with restriction enzyme digestions, sequencing, PCR, qPCR and more. Also available in 96-well format for high throughput applications.

Kit Specifications (Spin Columns)
Maximum Input
2 x 109 bacterial cells
Column Binding Capacity
25 µg
Average Yield*
Up to 20 µg
Time to Complete 10 Purifications
1 hour
Kit Specifications (96-well)
Maximum Input
2 x 109 bacterial cells
Well Binding Capacity
25 µg
Average Yield*
Up to 20 µg*
Time to Complete 10 Purifications
90 minutes

* Yield will vary depending on the type of sample processed

Storage Conditions and Product Stability
The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

 

 

Kit Components
Component Cat. 17900 (50 preps)
Resuspension Solution A 20 mL
Lysis Buffer P 18 mL
Solution BX 28 mL
Wash Solution A 18 mL
Elution Buffer B 30 mL
Proteinase K 12 mg
Spin Columns inserted into Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1
   
Component Cat. 17950 (96-well)
Resuspension Solution 60 mL
Lysis Solution 60 mL
Binding Solution 110 mL
Wash Solution 2 x 34 mL
Elution Buffer 50 mL
Proteinase K 50 mg
96-well Filter Plate 2
Adhesive Tape 4
Collection Plate 2
Elution Plate 2
Product Insert 1
Title Genotypic and Phenotypic Heterogeneity in Alicyclobacillus acidoterrestris: A Contribution to Species Characterization
Journal PLOS ONE. 2015.
Authors Antonio Bevilacqua, Monica Mischitelli, Valeria Pietropaolo, Emanuela Ciuffreda, Milena Sinigaglia, Maria Rosaria Corbo
Title Characterization and the influence of milk solids-not-fat on the bacteriocin produced by Lactococcus lactissubsp. lactis S20 isolated from Chinese sauerkraut, a traditional fermented vegetable
Journal Annals of Microbiology. 2015.
Authors Yih-Zhet Chin, Selvi Velu, Fatimah Abu Bakar, Mahmud-Ab-Rashid Nor-Khaizura
Title Development of a Multiple Loci Variable Number of Tandem Repeats Analysis (MLVA) to Unravel the Intra-Pathovar Structure of Pseudomonas syringae pv. actinidiae Populations Worldwide
Journal PLOS ONE. 2015.
Authors Serena Ciarroni, Lorenzo Gallipoli, Maria C. Taratufolo, Margi I. Butler, Russell T. M. Poulter, Christine Pourcel, Gilles Vergnaud, Giorgio M. Balestra, Angelo Mazzaglia
Title Molecular Epidemiology of Escherichia Coli in HIV-Positive Individuals in South west Nigeria
Journal International Archives of Medicine. 2015.
Authors Lawrence Ehis Okoror, Duna Christina Fashina, Titilayo Silifat Jimoh, Ezekiel Ayo Oisagah
Title Genome Sequence of Anoxybacillus flavithermus Strain AK1, a Thermophile Isolated from a Hot Spring in Saudi Arabia
Journal Genome Announcements. 2015.
Authors Khalil A, Sivakumar N, Qarawi S
Title Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda
Journal BioMed Research International. 2015.
Authors Denis Rwabiita Mugizi, Shaman Muradrasoli, Sofia Boqvist, Joseph Erume, George William Nasinyama, Charles Waiswa, Gerald Mboowa, Markus Klint, and Ulf Magnusson
Title Microarray-based identification of human antibodies against Staphylococcus aureus antigens
Journal Proteomics Clinical Applications. 2015.
Authors Peggy Kloppot, Martina Selle, Christian Kohler, Sebastian Stentze, Stephan Fuchs, Volkmar Liebscher, Elke Müller, Devika Kale, Knut Ohlsen, Barbara M. Bröker, Peter F. Zipfel, Barbara C. Kahl, Ralf Ehricht, Michael Hecker and Susanne Engelmann
Title Isolation of a significant fraction of non-phototroph diversity from a desert Biological Soil Crust.
Journal Frontiers in Microbiology. 2015.
Authors Ulisses Nunes da Rocha, Hinsby Cadillo-Quiroz, Ulas Karaoz, Lara Rajeev, Niels Klitgord, Sean Dunn, Viet Truong, Mayra Buenrostro, Benjamin Paul Bowen, Ferran Garcia-Pichel, Aindrila Mukhopadhyay, Trent Russell Northen and Eoin L Brodie.
Title Investigation of the Presence of Class 1, 2, 3 Integrons and Their Relationships with Antibiotic Resistance in Clinical Stenotrophomonas maltophilia Isolates.
Journal Mikrobiyol Bul. 2015.
Authors Egemen USTA, Cafer EROĞLU, Keramettin YANIK, Adil KARADAĞ, Akif Koray GÜNEY, Murat GÜNAYDIN.
Title Draft Genome Sequence of Burkholderia gladioli Strain UCDUG_ CHAPALOTE (Phylum Proteobacteria).
Journal Genome Announc. 2015.
Authors Cassandra L. Ettinger, Hanan R. Shehata, David Johnston-Monje, Manish N. Raizad, Jonathan A. Eisen.
Title Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda.
Journal BioMed Research International. 2014.
Authors Denis Rwabiita Mugizi, Shaman Muradrasoli, Sofia Boqvist, Joseph Erume, George William Nasinyama, Charles Waiswa, Gerald Mboowa, Markus Klint, and Ulf Magnusson.
Title Sequence Analysis of CRISPR Arrays of Erwinia amylovora isolates from Canada.
Journal ISHS Acta Horticulturae. 2014.
Authors A.I. Yagubi, A.J. Castle, A.M. Svircev.
Title Functional validation of putative toxin-antitoxin genes from the Gram-positive pathogen Streptococcus pneumoniae: phd-doc is the fourth bona-fide operon.
Journal Frontiers in Microbiology. 2014.
Authors Wai Ting Chan, Chew Chieng Yeo, Ewa Sadowy and Manuel Espinosa.
Title Insertion/Deletion-Based Approach for the Detection of Escherichia coli O157:H7 in Freshwater Environments.
Journal American Chemical Society. 2014.
Authors Shirley Y. Wong, Athanasios Paschos, Radhey S. Gupta, and Herb E. Schellhorn.
Title Genome Sequence of Growth-Improving Paenibacillus mucilaginosus Strain KNP414.
Journal Genome Announcements. 2013.
Authors Lu JJ, Wang JF, Hu XF.
Title Isolation and Molecular Characterization of Lactic Acid Bacteria Isolated from Fresh Fruits and Vegetables Using Nested PCR Analysis.
Journal British Microbiology Research Journal. 2013.
Authors Emerenini E, Afolabi O, Okolie P and Akintokun A
Title Characterizing metabolic interactions in a clostridial co-culture for consolidated bioprocessing.
Journal BMC Biotechnology. 2013.
Authors Salimi F, Mahadevan R.
Title Performance Evaluation and Bacterial Characterization of Membrane Bioreactors.
Journal Bioresource Technology. 2013.
Authors Khana SJ, Parveena F, Ahmada A, Hashmia I and Hankins N.
Title Plant growth promoting bacteria in Brachiaria brizantha.
Journal World Journal of Microbiology and Biotechnology. 2012.
Authors Silva MC, Figueiredo AF, Andreote FD, Cardoso EJ.
Title First Report of Leaf Spot of Sesame Caused by Xanthomonas sp. In the United States.
Journal Plant Disease. 2012.
Authors Isakeit T, Hassett B, and Ong K.
Title Heterologous expression and characterization of an N-acetyl-β-D-hexosaminidase from Lactococcus lactis ssp. lactis IL1403.
Journal Journal of Agricultural and Food Chemistry. 2011.
Authors Nguyen HA, Nguyen TH, Kren V, Eijsink VG, Haltrich D, Peterbauer CK.
Title Molecular Characterization of Clinical Isolates of Aeromonas Species from Malaysia.
Journal PLOS One. 2011.
Authors Puthucheary SD, Puah SM, Chua KH.
Title Re-examining the role of hydrogen peroxide in bacteriostatic and bactericidal activities of honey.
Journal Frontiers in Microbiology. 2011.
Authors Brudzynski K, Abubaker K, Laurent M, Castle A.
Title Macrolide resistance and In Vitro selection of resistance to antibiotics in Lactobacillus isolates.
Journal Journal of Microbiology. 2011.
Authors Drago L, Mattina R, Nicola L, Rodighiero V, De Vecchi E.