For the rapid preparation of BAC DNA

  • Fast and easy processing using alcohol precipitation or a rapid spin-column format
  • High yield of plasmid DNA – up to 1 µg from 3 mL of culture
  • Versatile protocol that allows different volume of input to be processed
Items Cat. # Size
BAC DNA MiniPrep Kit 18050 50 preps (1.5 mL)

BAC DNA Miniprep Kit

This kit is designed for the rapid preparation of BACs (bacterial artificial chromosomes) and other large DNA constructs from small batch cultures ofEscherichia coli. Purification of constructs up to 130 kb in size have been verified.Rapid alcohol precipitation or spin-column purification allows for the processing of multiple samples in 1 hour.​ The DNA is preferentially purified from other cellular components such as genomic DNA and RNA. Typical DNA recoveries range between 0.6 µg and 1 µg from 3.0 mL of bacterial cultures. The purified DNA is fully digestible with all restriction enzymes tested, and is fully compatible with bacterial transformation and sequencing to achieve 95-100% accuracy.

Kit Specifications
Input Culture Volume
Alcohol Precipitation: Up to 100 mL
Column Purification: Up to 5 mL
Column Binding Capacity
25 µg
Size of Plasmids Purified
Up to 130 kbp
Average Yield from 3 mL of Culture
0.6 - 1 µg
Time to Complete 10 Purifications
1 hour

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RNAse should be stored at -20°C upon arrival. All the reagents should remain stable for at least 1 year in their unopened containers.