ProteoSpin™ Total Protein Concentration and Detergent Clean-Up Mini Kit

For the rapid concentration and clean-up of protein samples

  • Concentrate protein samples and remove detergents at the same time
  • Rapid and convenient spin-column protocol is completed in about 20 minutes
  • Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20
Items Cat. # Size
ProteoSpin™ Total Protein Concentration and Detergent Clean-Up Mini Kit 23350 25 preps

ProteoSpin™ Total Protein Concentration and Detergent Clean-Up Mini Kit

This kit simultaneously removes detergents, salts, denaturants and other contaminants from protein samples while concentrating and buffer exchanging with a rapid and convenient spin column method in about 20 minutes. All types of detergents including ionic, non-ionic and zwitterionic detergents can be efficiently removed. Both free detergents and those bound to proteins are effectively removed. Unlike other commercially available kits that can only handle one detergent and often do not remove denaturants, this kit removes a variety of detergents and denturants that may be used during the purification of the protein sample while providing high protein recovery.

No further cleaning of the protein sample is required, and it can be used directly in a variety of downstream applications including mass spectrometry, SDS-PAGE, isoelectric focusing, and NMR spectroscopy.

 

 

Kit Specifications
Maximum Protein Input
200 μg
Maximum Protein Volume Input
500 μL
Protein Recovery
80-95%
% Detergent Removal
> 95%
Minimum Elution Volume
30 μL
Time to Process 10 Samples
20 minutes

Storage Conditions
Unopened solutions should be stored at room temperature. Once opened, the solutions should be stored at 4°C when not in use.

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